Cover Page
The handle
http://hdl.handle.net/1887/139216
holds various files of this Leiden
University dissertation.
Author: Lubbers, R.
Title: Complement biology in health and disease
Complement biology in health and disease
COLOPHON
Complement biology in health and disease ISBN 978-94-6416-268-4
Layout and design: Birgit Vredenburg | persoonlijkproefschrift.nl Printing: Ridderprint | www.ridderprint.nl
The research described in this thesis was carried out at the department of Rheumatology, Leiden University Medical Center, Leiden, the Netherlands.
Financial support by the Dutch Arthritis Society for the publication of this thesis is gratefully acknowledged.
Copyright © Rosalie Lubbers
All rights reserved. No part of this thesis may be reproduced, stored or transmitted in any way or by any means without the prior permission of the author, or when applicable, of the publishers of the scientific papers. All published material was reprinted with permission.
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Complement biology in health and disease Proefschrift
Ter verkrijging van
de graad van Doctor aan de Universiteit Leiden op gezag van rector magnificus Prof. Mr. C.J.J.M Stolker,
volgens besluit van College voor Promoties te verdedigen op donderdag 21 januari 2021
klokke 16:15 uur door Rosalie Lubbers geboren te Hardenberg
PROMOTORES
Prof. Dr. R.E.M. Toes Prof. Dr. T.W.J. HuizingaCO-PROMOTOR
Dr. L.A. TrouwPROMOTIECOMMISSIE
Prof. Dr. C. van KootenDr. I. Jongerius (Sanquin, Amsterdam) Prof. Dr. S. Rooijakkers (UMC Utrecht) Dr. F.J. Beurskens (Genmab, Utrecht)
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TABLE OF CONTENTS
Chapter 1 General introduction 9
Chapter 2 Complement component C1q is locally produced by isolated articular
chondrocytes 25
Chapter 3 Complement C3 cleavage by Cathepsin-L leads to formation of
C3a-desArg and is not essential for survival in a HAP-1 model cell line 45
Chapter 4 Complement component C1q as serum biomarker to detect active
tuberculosis 69
Chapter 5 Analysis of the endogenous complement regulator C1-inhibitor, the
product of SERPING1, in active tuberculosis 93
Chapter 6 Systemic and pulmonary C1q as biomarker of progressive disease in
experimental non-human primate tuberculosis 113
Chapter 7 Complex medical history of a lupus patient with a compound
heterozygous mutation in C1QC 139
Chapter 8 Carbamylation reduces capacity of IgG for hexamerisation and
complement activation 151
Chapter 9 Summary and general discussion 173
Addendum Nederlandse samenvatting 190
Curriculum Vitae 197
List of publications 198
