Cover Page
The handle
https://hdl.handle.net/1887/3158165
holds various files of this Leiden
University dissertation.
Author: Oliveira Paiva, A.M.
Title: New tools and insights in physiology and chromosome dynamics of Clostridioides
difficile
New tools and insights in physiology
and chromosome dynamics of
Clostridioides difficile
Colophon
Thesis The research described in this thesis was performed at Leiden University Medical Center, Department of Medical Microbiology, Leiden, The Netherlands
Financial support Work in the group of WKS was supported by a Vidi Fellowship (864.10.003) of the Netherlands Organization for Scientific Research (NWO) and a Gisela Thier Fellowship from the Leiden University Medical Center.
Cover António Paiva design. Microscopy picture of C. difficile strain RD16, harbouring HupA-HaloTag, induced at mid-exponential growth phase with 200 ng/ml anhydrotetracycline for 1 hour, imaged with the A4 filter cube (340-380 nm excitation and 450-490 nm emission) for the coumarin substrate (at 150 nM) visualization, on a Leica AF6000 microscope with LAS AF software. Microscopy performed by Roxanne Douwes.
Thesis design Ana Paiva
Print Proefschriftmaken (www.proefschriftmaken.nl)
ISBN/EAN
Copyright © 2021, Ana Margarida Oliveira Paiva. All rights reserved. The copyright of the published articles has been transferred to the respective journals or publishers. No part of this publication may be reproduced, stored in a retrieval system, or transmitted in any form or by any means without prior permission of the author, the respective journal or publisher.
New tools and insights in physiology
and chromosome dynamics of
Clostridioides difficile
Proefschrift tĞƌǀĞƌŬƌŝũŐŝŶŐǀĂŶĚĞŐƌĂĂĚǀĂŶĚŽĐƚŽƌĂĂŶĚĞhŶŝǀĞƌƐŝƚĞŝƚ>ĞŝĚĞŶ͕ŽƉŐĞnjĂŐǀĂŶƌĞĐƚŽƌ ŵĂŐŶŝĨŝĐƵƐƉƌŽĨ͘Ěƌ͘ŝƌ͘,͘ŝũů͕ǀŽůŐĞŶƐďĞƐůƵŝƚǀĂŶŚĞƚĐŽůůĞŐĞǀŽŽƌƉƌŽŵŽƚŝĞƐƚĞ ǀĞƌĚĞĚŝŐĞŶŽƉϯϬŵĂĂƌƚϮϬϮϭŬůŽŬŬĞϭϱ͘ϬϬƵƵƌ doorAna Margarida Oliveira Paiva geboren ƚĞ>ĞŝƌŝĂ;WŽƌƚƵŐĂůͿ
Promotor: Prof. Dr. E. J. Kuijper
Co-promotor: Dr. W.K. Smits
Leden van de promotiecommissie:
Prof. Dr. T.H.M. Ottenhoff Prof. Dr. A. Briegel
Dr. P. Salgado ;EĞǁĐĂƐƚůĞhŶŝǀĞƌƐŝƚLJͿ Dr. M. Lamers
To my Mom and Dad
Thank you for always believing in me
Table of contents
Chapter 1 General Introduction and Thesis Outline 9
Chapter 2 The signal sequence of the abundant extracellular metalloprotease PPEP-1 can be used to secrete synthetic reporter proteins in
Clostridioides difficile
44
Chapter 3 The C-terminal domain of Clostridioides difficile TcdC is exposed on the bacterial cell surface
66
Chapter 4 Identification of the unwinding region in the Clostridioides difficile chromosomal origin of replication
94
Chapter 5 The bacterial chromatin protein HupA can remodel DNA and associates with the nucleoid in Clostridium difficile
130
Chapter 6 Shedding light on Clostridioides difficile: practical observations on the use of fluorescent reporter systems
174
Chapter 7 General Discussion 218
English Summary 239
Dutch Summary (Samenvatting) 243
Portuguese Summary (Sumário) 247
List of Publications 251
Curriculum Vitae 253
