Nedialkova, D.D.
Citation
Nedialkova, D. D. (2010, June 23). Exploring regulatory functions and enzymatic activities in the nidovirus replicase. Retrieved from
https://hdl.handle.net/1887/15717
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Exploring regulatory functions and
enzymatic activities in the nidovirus replicase
Danny Nedialkova
Exploring
regulatory functions and enzymatic activities in the nidovirus replicase
Proefschrift
ter verkrijging van
de graad van Doctor aan de Universiteit Leiden, op gezag van Rector Magnificus prof.mr. P.F. van der Heijden,
volgens besluit van het College voor Promoties te verdedigen op woensdag 23 juni 2010
klokke 11.15 uur door
Danny Doncheva Nedialkova geboren te Sofia, Bulgarije
in 1983
Promotores:
Prof. Dr. A.E. Gorbalenya
Overige leden: Prof. Dr. W. J. M. Spaan
Prof. Dr. N. Tautz (University of Lübeck) Dr. R.J. de Groot (Universiteit Utrecht)
The research described in this thesis was carried out at the Department of Medical Mi- crobiology of the Leiden University Medical Center, Leiden, The Netherlands, and was financially supported by grant 700.52.306 from the Council for Chemical Sciences of the Netherlands Organization for Scientific Research (NWO-CW).
“Science is not about building a body of known ‘facts’.
It is a method for asking awkward questions and subjecting them to a reality-check, thus avoiding the human tendency to believe whatever makes us feel good.”
Terry Pratchett
За мама
CONteNts
CHAPTER 1 General introduction 9
CHAPTER 2 Regulation of the plus-strand RNA virus replicative cycle:
putting it together by taking it apart
31
CHAPTER 3 Site-directed mutagenesis of the nidovirus replicative endoribonuclease NendoU exerts pleiotropic effects on the arterivirus life cycle
81
CHAPTER 4 Biochemical characterization of arterivirus nonstructural protein 11 reveals the nidovirus-wide conservation of a replicative endoribonuclease
101
CHAPTER 5 Arterivirus subgenomic mRNA synthesis and virion biogenesis depend on the multifunctional nsp1 autoprotease
127
CHAPTER 6 Arterivirus nsp1 modulates the accumulation of minus- strand templates to control the relative abundance of viral mRNAs
149
CHAPTER 7 Expression and purification of recombinant equine arteritis virus nonstructural protein 1
179
CHAPTER 8 General discussion 203
SUMMARy 229
SAMENvATTiNG 231
CURRiCULUM viTAE 237
