Exploring regulatory functions and enzymatic activities in the nidovirus replicase Nedialkova, D.D.

Wild-type nsp1-HN-ap could be efficiently purified by metal affinity chromatography with Talon resin (charged with cobalt ions), albeit in the presence of a number of abun-

This approach was em- ployed in the research outlined in Chapters 3 and 4 to gain more insight into the role of the conserved NendoU domain in the EAv replicative cycle and to

These data identified nsp1 as a multifunctional replicase subunit with critical functions in at least three consecutive key processes in the EAv replicative cycle:

De autoproteolytische klieving van nsp1 van de replicase polyproteïnen wordt uitgevoerd door een C-terminaal papaine-achtig cysteine proteinase domein (PCPβ), en klieving tussen

As part of this program, she completed research internships at the intercollegiate Faculty of Biotechnology at the University of Gdansk (Poland) and the De- partment of

Viewed in the context of the discontinuous minus-strand extension model, the concomitant changes in minus-strand and plus-strand RNA accumulation levels associated with mutations

Peripheral blood cells were stained with HLA-A2.1 tetramers containing the tyrosinase368–376 peptide followed by staining with a panel of lineage antibodies, as described in

Blades and blade fragments seem to have been especially used for longitudinal motions, mainly on plant material (7/12). Flake and flake fragments are used in different motions on