Cover Page
The handle http://hdl.handle.net/1887/39949 holds various files of this Leiden University dissertation.
Author: Pont, M.J.
Title: Discovery of minor histocompatibility antigens as targets for immunotherapy
Issue Date: 2016-06-07
DISCOVERY OF
MINOR HISTOCOMPATIBILITY ANTIGENS AS TARGETS FOR IMMUNOTHERAPY
Margot Pont
Discovery of minor histocompatibility antigens as targets for immunotherapy
© 2016 Margot Pont Amsterdam
Cover image: Kristian Bolk, Mt. Rainier National Park rainforest Layout: Margot Pont, based on design by Liset Westera Printing: Ipskamp, Enschede, The Netherlands
ISBN: 978-94-028-0175-0
The research described in this thesis was performed at the Department of Hematology of the Leiden University Medical Center in Leiden, the Netherlands and was financially supported by the Dutch Cancer Society (UL 2010-4748).
Printing of this thesis was financially supported by Boehringer Ingelheim, Chipsoft, Greiner Bio-One and BD Biosciences.
Discovery of
minor histocompatibility antigens as targets for immunotherapy
Proefschrift
ter verkrijging van
de graad van Doctor aan de Universiteit Leiden, op gezag van Rector Magnificus prof.mr. C.J.J.M. Stolker,
volgens besluit van het College voor Promoties te verdedigen op dinsdag 7 juni 2016
klokke 15:00 uur door
Margot Jooske Pont
geboren te Leidschendam
in 1986
Promotor:
Prof. dr. J.H.F. Falkenburg
Co-promotor:
Dr. M. Griffioen
Promotiecommissie:
Prof. dr. S.H. van der Burg
Prof. dr. T.C. de Gruijl, VU Medisch Centrum, Amsterdam
Dr. T. Mutis, VU Medisch Centrum, Amsterdam
wees niet bang voor al te grote dromen ga als je het zeker weet, en als je aarzelt wacht hoe ijdel zijn de dingen die je je hebt voorgenomen
het mooiste overkomt je, het minste is bedacht
- Freek de Jonge, ‘Kijk, Dat Is Freek’, 2011
To those who made me who I am
Chapter 1
Chapter 2
Chapter 3
Chapter 4
Chapter 5
Chapter 6
Appendices
9
29
73
89
107
139
160 167 169 170
Table of Contents
General introduction and Aim of the study
Microarray gene expression analysis to evaluate cell type specific expression of targets relevant for immunotherapy of hematological malignancies
LB-ARHGDIB-1R as novel minor histocompatibility antigen for therapeutic application
Minor histocompatibility antigen LB-TTK-1D is encoded by an alternative transcript that is degraded by nonsense mediated decay
Integrated whole genome and transcriptome analysis identified a therapeutic minor histocompatibility antigen in a splice variant of ITGB2
Summary and General discussion
Nederlandse samenvatting Dankwoord
Curriculum vitae List of publications
