University of Groningen
Physiological Consequences of protein translocation stress in Bacillus subtilis Bernal-Cabas, M.
DOI:
10.33612/diss.143818857
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Publication date: 2020
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Bernal-Cabas, M. (2020). Physiological Consequences of protein translocation stress in Bacillus subtilis. https://doi.org/10.33612/diss.143818857
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I
Physiological consequences of protein translocation
stress in Bacillus subtilis
II
The PhD research described in this thesis was performed in the laboratory of Molecular Bacteriology, Department of Medical Microbiology, of the University of Groningen and the University Medical Center Groningen, the Netherlands.
This research was financially supported by the by the People Programme (Marie Skłodowska-Curie Actions) of the European Union’s Horizon 2020 Programme under REA grant agreement no. 642836, and the Graduate School of Medical Sciences of the University of Groningen.
Printing of this thesis was supported by the Graduate School of Medical Sciences of the University of Groningen.
Dissertation of the University of Groningen
Cover photo: The stressed membrane by Dr. Suruchi Nepal Printing: Ipskamp Printing, Enschede
Copyright © María Margarita Bernal-Cabas, 2020. All rights reserved. No part of this thesis may be reproduced, stored in a retrieval system, or transmitted in any form or by any means, without prior written permission of the author.
III
Physiological consequences of protein translocation stress
in Bacillus subtilis
PhD thesis
to obtain the degree of PhD at the University of Groningen
on the authority of the
Rector Magnificus Prof. C. Wijmenga and in accordance with the decision by the College of Deans. This thesis will be defended in public on Wednesday 4 November 2020 at 09:00 hours
by
María Margarita Bernal-Cabas born on 13 February 1988
IV
Supervisor Prof. J.M. van Dijl Co-supervisor Dr. G. Buist
Assessment committee Prof. C. Robinson Prof. A.J.M Driessen Prof. O.P Kuipers
V
Paranymphs
Dr. Giorgio Gabarrini Drs. Marina López-Álvarez Dr. Suruchi Nepal
VII
Table of contents
Chapter 1
General introduction and scope of this thesis 1Chapter 2
Functional association of the stress-responsive LiaH protein and the minimal TatAyCy protein translocase in Bacillussubtilis
Published in Biochimica et Biophysica Acta - Molecular Cell
Research. 1867, 118719 (2020)
29
Chapter 3
In the Cave of Wonders – 15N metabolic labelling provides newinsights in cell physiology during twin-arginine translocation in
Bacillus subtilis
Manuscript to be submitted
65
Chapter 4
Double trouble: Bacillus depends on a functional Tat machinery to avoid severe oxidative stress and starvation upon entry into a NaCl-depleted environmentManuscript under revision at Biochimica et Biophysica Acta -
Molecular Cell Research
105
Chapter 5
Ariadne’s Thread in the Analytical Labyrinth of Membrane Proteins – Integration of Targeted and Shotgun Proteomics for Global Absolute Quantification of Membrane ProteinManuscript published in Analytical Chemistry. 91, 11972–11980 (2019).
139
Chapter 6
Membrane modulation of super-secreting “midiBacillus” expressing the major Staphylococcus aureus antigen – a mass-spectrometry based absolute quantification approach Manuscript published in Frontiers in Bioengineering andBiotechnology. 8, 1–15 (2020).
161
Chapter 7
Summary and future perspectives 195Chapter 8
Dutch summary (Nederlandse samenvattig) 207Chapter 9
I. Acknowledgements II. List of publications III. Biography220 228 229
