University of Groningen
Physiological Consequences of protein translocation stress in Bacillus subtilis Bernal-Cabas, M.
DOI:
10.33612/diss.143818857
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Publication date: 2020
Link to publication in University of Groningen/UMCG research database
Citation for published version (APA):
Bernal-Cabas, M. (2020). Physiological Consequences of protein translocation stress in Bacillus subtilis. https://doi.org/10.33612/diss.143818857
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Propositions accompanying the thesis
Physiological consequences of protein translocation stress in Bacillus subtilis
1. Deeper understanding of the physiological consequences of production stress in
bacterial cell factories is of vital importance for the development of the next generation of super secreting strains (This thesis).
2. The intricate balance between heterologous protein production and membrane
homeostasis can either be tipped towards high product yields or cell death (Chapter 1). 3. The minimal Tat translocase TatAyCy is not so minimal after all (Chapter 2).
4. Bacteria can also suffer from ‘burnout’ (Chapter 3).
5. Arginine has an important role in Tat-mediated protein secretion that goes beyond the RR-motif in the signal peptide (Chapters 3 and 4).
6. The role of the TatAyCy translocase in the physiology of Bacillus subtilis has been hidden in plain sight (Chapter 4).
7. Absolute membrane protein quantification opens up a new chapter in the history of
Bacillus subtilis by providing deeper insights into membrane proteome dynamics in
response to stress (Chapters 5 and 6).
8. Understanding membrane protein modulation upon high-level protein secretion will enable a better resource allocation (Chapter 6).
9. Proteomics always tells a story, no matter what the result is (This thesis).
10. Bistability is an underrated phenomenon in high-level protein production (This thesis). 11. "It is our choices ... that show what we truly are, far more than our abilities." - J.K.
