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University of Groningen Less is more: Genome-reduced Bacillus subtilis for protein production Aguilar Suarez, Rocio

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University of Groningen

Less is more: Genome-reduced Bacillus subtilis for protein production

Aguilar Suarez, Rocio

DOI:

10.33612/diss.146898256

IMPORTANT NOTE: You are advised to consult the publisher's version (publisher's PDF) if you wish to cite from

it. Please check the document version below.

Document Version

Publisher's PDF, also known as Version of record

Publication date:

2020

Link to publication in University of Groningen/UMCG research database

Citation for published version (APA):

Aguilar Suarez, R. (2020). Less is more: Genome-reduced Bacillus subtilis for protein production.

University of Groningen. https://doi.org/10.33612/diss.146898256

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The work described in this dissertation was performed in i) the Molecular Bacteriology research group part of the Department of Medical Microbiology of the University of Groningen-University Medical Center Groningen, the Netherlands, as well as ii) Department of Microbial Proteomics, Centre of Functional Genomics of Microbes, University of Greifswald, Germany, and iii) Institute for Systems Biotechnology, Saarland University, Germany. This PhD research was financially supported by Conacyt.

Printing of this thesis was supported by the University of Groningen and the Graduate School of Medical Sciences.

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Less is more: Genome-reduced Bacillus subtilis

for protein production

PhD thesis

to obtain the degree of PhD at the

University of Groningen

on the authority of the

Rector Magnificus Prof. C. Wijmenga

and in accordance with

the decision by the College of Deans.

This thesis will be defended in public on

Wednesday 9 December 2020 at 14:30 hours

by

Rocio Aguilar Suarez

born on 10 September 1988

in Naucalpan de Juarez, Mexico

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Supervisor

Prof. J.M. van Dijl

Co-supervisor Dr. G. Buist Assessment committee Prof. K. Yoshida Prof. G.J.W. Euverink Prof. D.J. Scheffers

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Paranymphs

B. Prajapati, MSc.

S. Piersma, dr.

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Table of Contents

Chapter 1

Introduction

Scope of this dissertation

8

1

Chapter 2

Toward a genome-reduced Bacillus cell factory for ‘difficult proteins’

11

Chapter 3

Membrane modulation of midiBacillus-II: absolute protein quantification

31

Chapter 4

A redirected protein secretion stress response in the genome-engineered

midiBacillus-II

57

Chapter 5

Characterisation of midiBacillus-I for biotechnological application

85

Chapter 6

Summary and future work.

Nederlandse samenvatting en toekomstperspectieven

107

115

References

Abbreviations

Acknowledgements

List of publications

Biography

124

132

133

138

139

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