University of Groningen
Snap Shots of Bacterial Adaptation
Prajapati, Bimal
DOI:
10.33612/diss.145910301
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Publication date: 2020
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Prajapati, B. (2020). Snap Shots of Bacterial Adaptation. University of Groningen. https://doi.org/10.33612/diss.145910301
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Snap Shots of Bacterial Adaptation
The research described in this thesis was conducted at the Molecular Bacteriology lab at the Department of Medical Microbiology of the University Medical Center Groningen and the University of Groningen, the Netherlands. This research was funded by the Graduate School of Medical Sciences and the Ubbo Emmius Fund.
Printing of this thesis was financially supported by the Graduate School of Medical Sciences of the University of Groningen and Groningen University Library.
Snap Shots of Bacterial Adaptation Dissertation of the University of Groningen ISBN: 978-94-6421-128-3
Cover photo: Voronoi treemaps comparing the transcriptome of the B. subtilis
tat-mutant bacteria to the wild-type strain 168 during the lysis phase and
recovery phase when grown in Lysogeny Broth without NaCl. Printed by: Ipskamp drukkers, Enschede
Copyright © Bimal Prajapati, 2020
All rights reserved. No part of this thesis may be reproduced, stored in a retrieval system or transmitted in any form or by any means without prior written
Snap Shots of Bacterial Adaptation
PhD thesis
to obtain the degree of PhD at the University of Groningen
on the authority of the
Rector Magnificus Prof. C. Wijmenga and in accordance with
the decision by the College of Deans. This thesis will be defended in public on Wednesday 25 November 2020 at 12:45 hours
by
Bimal Prajapati
born on 24 September 1988 in Kathmandu, Nepal
Supervisors
Prof. J.M. van Dijl Prof. A.W. Friedrich
Assessment Committee
Prof. C.R. Harwood Prof. H.J. Busscher Prof. J. Kok
Paranymphs
Tim Stobernack Marines du Teil Espina Marina López-Álvarez
In loving memory of my mother Bijayaswori Prajapati
Table of Contents
Chapter 1 Introduction and scope of the thesis 9 Chapter 2 Double trouble: Bacillus depends on a functional
Tat machinery to avoid severe oxidative stress and starvation upon entry into a NaCl-depleted environment
35
Chapter 3 Conditional essentiality of the small RNA S313 for expression of the elemental iron transport system
efeUOB in Bacillus subtilis and resistance to
cefuroxime
77
Chapter 4 In the Cave of Wonders – 14N/15N metabolic
labelling provides new insights in cell physiology during twin-arginine translocation in Bacillus
subtilis
121
Chapter 5 Induction of phages differentiates the adaptative response to subinhibitory ciprofloxacin exposure in clinical S. aureus USA300 isolates
171
Chapter 6 Summary, Conclusion and Future Perspectives 199 Appendix Nederlandse Samenvatting 215
Acknowledgements 225
