University of Groningen
The impact of genotoxic stress on protein homeostasis
Huiting, Wouter
DOI:
10.33612/diss.168249330
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Publication date: 2021
Link to publication in University of Groningen/UMCG research database
Citation for published version (APA):
Huiting, W. (2021). The impact of genotoxic stress on protein homeostasis: a study on an emerging theme and its relevance for age-related degeneration. University of Groningen.
https://doi.org/10.33612/diss.168249330
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the
impact
of
genotoxic stress
A study on an emerging theme
and its relevance for
age-related degeneration
The research described in this thesis was conducted at the University of Groningen, University Medical Center Groningen, The Netherlands, at the Department of Biomedical Sciences of Cells and Systems, and within the European Research Institute for the Biology of Ageing (ERIBA), between 2016 and 2021. The research was financially supported by the Netherlands Organisation for Scientific Research (NWO). Printing of this thesis was financially supported by the University of Groningen.
Cover Design: Sita Dijkstra, @zonniger INSTAGRAM Thesis Layout: Els Kuiper, www.elskuiper.nl Thesis printed by: Gildeprint, www.gildeprint.nl
Copyright © 2021 by Wouter Huiting. All rights reserved. No part of this thesis may be reproduced, stored in a retrieval system, or transmitted in any form or by any means without permission of the author.
The impact of genotoxic stress on
protein homeostasis
A study on an emerging theme and its relevance for age-related degeneration
Proefschrift
ter verkrijging van de graad van doctor aan de Rijksuniversiteit Groningen
op gezag van de
rector magnificus prof. dr. C. Wijmenga en volgens besluit van het College voor Promoties
De openbare verdediging zal plaatsvinden op Maandag 10 mei 2021 om 11.00 uur
door
Wouter Huiting
Geboren op 10 juni 1990 te Nieuwegein
SUPERVISORS
Dr. Steven BerginkProf. dr. Harm H. Kampinga
ASSESSMENT COMMITTEE
Prof. dr. Ineke BraakmanProf. dr. Zuzana Storchová Prof. dr. Marcel van Vugt
PARANYMPHS
Suzanne Dekker Els KuiperChapter 1
Introduction
9
Chapter 2
Impaired function of ATM, ATR, and DNA topoisomerases triggers the aggregation of a metastable subproteome through an overload of protein quality control systems
43
Chapter 3
Differential extraction of detergent-insoluble protein aggregates from cultured mammalian cells - a benchtop centrifuge method
79
Chapter 4
Loss of protein homeostasis is a common pathological mechanism in DNA damage response-compromised Caenorhabditis elegans
93 Chapter 5 Discussion 115 Bibliography 131 Appendix
List of abbreviations used English summary
Nederlandse samenvatting Acknowledgements
Short CV with a list of publications
167 171 177 183 191
