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University of Groningen

Deciphering the antiviral potential of tomatidine towards mosquito-borne viral infections

Troost-Kind, Berit

DOI:

10.33612/diss.161786279

IMPORTANT NOTE: You are advised to consult the publisher's version (publisher's PDF) if you wish to cite from

it. Please check the document version below.

Document Version

Publisher's PDF, also known as Version of record

Publication date:

2021

Link to publication in University of Groningen/UMCG research database

Citation for published version (APA):

Troost-Kind, B. (2021). Deciphering the antiviral potential of tomatidine towards mosquito-borne viral

infections. University of Groningen. https://doi.org/10.33612/diss.161786279

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Deciphering the antiviral

potential of tomatidine

towards

mosquito-borne viral

infections

Berit H. Troost-Kind

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The research presented in this thesis was conducted at the Department of

Medical Microbiology, University Medical Center Groningen, University of

Groningen, Groningen, the Netherlands.

Part of the work presented in this thesis has been suppoted by the Graduate

School of Medical Sciences, University of Groningen and the Jan Kornelis de

Cock Foundation (Groningen).

Printing of this thesis has been financially supported by the University of

Groningen and the Groningen University Institute for Drug Exploration

(GUIDE).

Copyright © 2021 Berit Helen Troost-Kind

All rights reserved. No part of this publication may be reproduced or

transmitted in any form or by any means without written permission of the

author and, when appropriate, the publisher holding the copyrights of the

published articles.

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Deciphering the antiviral potential

of tomatidine towards

mosquito-borne viral infections

PhD thesis

to obtain the degree of PhD at the University of Groningen

on the authority of the Rector Magnificus Prof. C. Wijmenga

and in accordance with the decision by the College of Deans. This thesis will be defended in public on Wednesday 31 March 2021 at 9:00 hours

by

Berit Helen Troost-Kind

born on 8 April 1991 in Remscheid, Germany

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Supervisor

Prof. J.M. Smit

Co-supervisor

Dr. I.A. Rodenhuis-Zybert

Assessment Committee

Prof. H.H. Kampinga Prof. C.A.H.H. Daemen Prof. N. Zitzmann

Paranymphs

Ellen Bouma

Federica Sicca

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Chapter 1

General introduction and scope of the thesis

9

Chapter 2

Recent advances in antiviral drug development towards

dengue virus

37

Chapter 3

Tomatidine, a novel antiviral compound towards dengue virus

63

Chapter 4

Tomatidine, a natural steroidal alkaloid shows antiviral activity

towards chikungunya virus in vitro

87

Chapter 5

Tomatidine inhibits a post-entry stage of the chikungunya

virus replication cycle

113

Chapter 6

The antiviral efficacy of tomatidine towards dengue virus in

vivo

139

Chapter 7

Summarizing discussion and future perspectives

151

Nederlandse samenvatting

169

Acknowledgements

175

Appendix

Curriculum vitae

List of publications

181

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