University of Groningen
Macrophage-matrix interactions: orchestrating the fibrotic response?
Vasse, Gwenda
DOI:
10.33612/diss.170746933
IMPORTANT NOTE: You are advised to consult the publisher's version (publisher's PDF) if you wish to cite from it. Please check the document version below.
Document Version
Publisher's PDF, also known as Version of record
Publication date:
2021
Link to publication in University of Groningen/UMCG research database
Citation for published version (APA):
Vasse, G. (2021). Macrophage-matrix interactions: orchestrating the fibrotic response?. University of Groningen. https://doi.org/10.33612/diss.170746933
Copyright
Other than for strictly personal use, it is not permitted to download or to forward/distribute the text or part of it without the consent of the author(s) and/or copyright holder(s), unless the work is under an open content license (like Creative Commons).
Take-down policy
If you believe that this document breaches copyright please contact us providing details, and we will remove access to the work immediately and investigate your claim.
Downloaded from the University of Groningen/UMCG research database (Pure): http://www.rug.nl/research/portal. For technical reasons the number of authors shown on this cover page is limited to 10 maximum.
MACROPHAGE – MATRIX INTERACTIONS:
ORCHESTRATING THE FIBROTIC RESPONSE?
GWENDA F. VASSE
The work described in this thesis was funded by the Graduate School of Medical Sciences and performed at the Department of Biomedical Engineering of the University Medical Center Groningen and at the Department of Pharmacokinetics, Toxicology and Targeting and the Department of Molecular Pharmacology of the University of Groningen, The Netherlands.
Printing of this thesis was financially supported by the University of Groningen, the Graduate School of Medical Sciences and the Netherlands society for Biomaterials and Tissue Engineering (NBTE).
Cover design: Gwenda F. Vasse Layout: Gwenda F. Vasse Printed by: Proefschrift-aio.nl
Copyright © 2021 Gwenda F. Vasse
All rights reserved. No part of this thesis may be reproduced or transmitted in any form or by any means without prior permission of the author.
Macrophage – matrix interactions:
orchestrating the fibrotic response?
Proefschrift
ter verkrijging van de graad van doctor aan de Rijksuniversiteit Groningen
op gezag van de
rector magnificus prof. dr. C. Wijmenga en volgens besluit van het College voor Promoties.
De openbare verdediging zal plaatsvinden op maandag 7 juni 2021 om 12.45 uur
door
Gwenda Fransien Vasse
geboren op 5 juli 1993 te Hardenberg
Promotores Dr. P. van Rijn
Prof. dr. B.N. Melgert
Beoordelingscommissie Prof. dr. C.V.C. Bouten Prof. dr. G. van den Bogaart Prof. dr. R.A. Bank
Paranimfen Laís Ribovski Fleur Ruijne
TABLE OF CONTENTS
Chapter 1 13
Introduction and Aim of this Thesis
Chapter 2 21
Macrophage–Stroma Interactions in Fibrosis: Biochemical, Biophysical, and Cellular Perspectives
Chapter 3 51
Collagen Morphology Influences Macrophage Shape and Marker Expression in Vitro
Chapter 4 73
Collagen Type I Alters the Proteomic Signature of Macrophages in a Collagen Morphology-Dependent Manner
Chapter 5 95
Preservation of Higher Levels of Denatured Collagen in Lung Tissue from Patients with Idiopathic Pulmonary Fibrosis Compared to Controls after Decellularization
Chapter 6 107
Single-Cell Reactomics: Real-Time Single-Cell Activation Kinetics of Optically Trapped Macrophages
Chapter 7 125
Summary, General Discussion, and Future Perspectives
Appendices 135 Samenvatting
Acknowledgements About the Author List of Publications
