University of Groningen
G23 peptide-mediated delivery of biodegradable nanocarriers across an in vitro blood-brain
barrier model
de Jong, Edwin
DOI:
10.33612/diss.132284892
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Publication date: 2020
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Citation for published version (APA):
de Jong, E. (2020). G23 peptide-mediated delivery of biodegradable nanocarriers across an in vitro blood-brain barrier model. University of Groningen. https://doi.org/10.33612/diss.132284892
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G23 peptide-mediated delivery of
biodegradable nanocarriers across
an in vitro blood-brain barrier model
Edwin de Jong
2020
The research presented in this thesis was conducted at the Department of Cell Biology and the Department of Biomedical Engineering, Faculty of Medical Sciences, University Medical Center Groningen, University of Groningen, the Netherlands.
The work in this thesis is part of the NWO Talent Programme Vidi, awarded to Dr. I.S. Zuhorn, with project number 12950, which is financed by the Dutch Research Council (NWO-TTW).
The author acknowledges the financial support for printing of this thesis by the Graduate School of Medical Sciences of the University of Groningen.
Layout by Douwe Oppewal Printed by Ipskamp Printing
© 2020 by Edwin de Jong, the Netherlands.
All rights reserved. No parts of this thesis may be reproduced, stored in a retrieval system or transmitted in any form or by any means without prior written permission of the author and the publisher holding the copyright of the published articles.
G23 peptide-mediated delivery of
biodegradable nanocarriers across
an in vitro blood-brain barrier model
Proefschrift
ter verkrijging van de graad van doctor aan de
Rijksuniversiteit Groningen
op gezag van de
rector magnificus prof. dr. C. Wijmenga
en volgens besluit van het College voor Promoties.
De openbare verdediging zal plaatsvinden op
woensdag 23 september 2020 om 14.30 uur
door
Edwin de Jong
geboren op 18 augustus 1987
te Opsterland
4
Promotores
Dr. I.S. Zuhorn
Prof. dr. S.C.D. van IJzendoorn
Beoordelingscommissie
Prof. dr. R. Brock
Prof. dr. E. Mastrobattista Prof. dr. K. Poelstra
5
Paranimfen
Jolanda Jongsma Wendy Oost
7
9
CONTENTS
Chapter 1 General introduction and scope of the thesis 11
Chapter 2 Robust formation of biodegradable polymersomes by direct hydration 25
Chapter 3 A filter-free blood-brain barrier model to quantitatively study 43 transendothelial delivery of nanoparticles by fluorescence spectroscopy
Chapter 4 THR transferrin receptor-targeting peptide is ineffective in 65 promoting transcytosis of PEG-P(CL-g-TMC) polymersomes
across the blood-brain barrier
Chapter 5 Cell-penetrating peptide characteristics of the G23 peptide 79
Chapter 6 Summary and perspectives 97
Nederlandse samenvatting 105
Dankwoord 111
