The handle http://hdl.handle.net/1887/18690 holds various files of this Leiden University dissertation.
Author: Selman, Maurice Henricus Johannes
Title: Glycosylation analysis of immunoglobulin G : method development and clinical applications
Issue Date: 2012-04-17
Glycosylation analysis of immunoglobulin G:
method development and clinical applications
Maurice Henricus Johannes Selman
published material was reprinted with permission.
The work presented in this thesis was performed at the Biomolecular Mass Spectrometry Unit, Department of Parasitology from the Leiden University Medical Center.
The studies described in this thesis were financially supported by Hoffmann la Roche.
The printing of this thesis was financially supported by Hoffmann la Roche
Cover design: Dirk-Jan de Graaf
Layout and printing: Off Page, www.offpage.nl
Glycosylation analysis of immunoglobulin G:
method development and clinical applications
Proefschrift
ter verkrijging van
de graad van Doctor aan de Universiteit Leiden,
op gezag van de Rector Magnificus Prof.mr. P.F. van der Heijden, volgens besluit van het College voor Promoties
te verdedigen op dinsdag 17 april 2012 klokke 16:15 uur
door
Maurice Henricus Johannes Selman
geboren te Heteren op 12 april 1980
Dr. M. Wuhrer Overige leden:
Prof. Dr. M. Yazdanbakhsh Prof. Dr. P.E. Slagboom Prof. Dr. H.S. Overkleeft
Prof. Dr. G.J. de Jong (Utrecht University, Departement of Biomedical Analysis) Prof. Dr. R. Geyer (Institute of Biochemistry, Justus-Liebig-University Giessen, Germany) Dr. N. Engler (F. Hoffmann - La Roche Ltd, Basel, Switzerland)
Table of contents
Chapter 1 IgG glycosylation analysis 7
Method development
Chapter 2 Fc specific IgG glycosylation profiling by robust nano-reversed 25 phase HPLC-MS using a sheath-flow ESI sprayer interface
Chapter 3 Cotton HILIC SPE microtips for micro-scale purification and 45 enrichment of glycans and glycopeptides
Chapter 4 Immunoglobulin G glycopeptide profiling by matrix-assisted laser 65 desorption ionization Fourier transform ion cyclotron resonance mass spectrometry
Chapter 5 MALDI-TOF-MS analysis of sialylated glycans and glycopeptides 87 using 4-chloro-α-cyanocinnamic acid matrix
Applications
Chapter 6 Changes in antigen-specific IgG1 Fc N-glycosylation upon 115 influenza and tetanus vaccination
Chapter 7 IgG Fc N-glycosylation changes in Lambert-Eaton myasthenic 135 syndrome and myasthenia gravis
Chapter 8 General discussion 155
Addendum Reference list 165
Summary 172
Samenvatting 175
Abbreviations 178
Acknowledgements 180
Curriculum Vitae 182
List of publications 183
