Angiogenesis, proteases and angiogenic factors during the inception of pregnancy. Crucial contributors or trivial bystanders?

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(1)Angiogenesis, proteases and angiogenic factors during the inception of pregnancy. Crucial contributors or trivial bystanders? Plaisier, G.M.. Citation Plaisier, G. M. (2008, May 7). Angiogenesis, proteases and angiogenic factors during the inception of pregnancy. Crucial contributors or trivial bystanders?. Retrieved from https://hdl.handle.net/1887/12861 Version:. Corrected Publisher’s Version. License:. Licence agreement concerning inclusion of doctoral thesis in the Institutional Repository of the University of Leiden. Downloaded from:. https://hdl.handle.net/1887/12861. Note: To cite this publication please use the final published version (if applicable)..

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(13) $IBQUFS. I&.7&$1PTJUJWFDPOUSPMTSFTVMUFEJOBCVOEBOUTJHOBMTETD%/"FODPEJOHGPS..1 ..1BOE..1 D%/"PGIVNBOFOEPNFUSJBMTUSPNBMDFMMTGPS..1BOED%/" PG)5DFMMTGPS..1 5IFFYQSFTTJPOPGBDUJWF..1TXBTDPO¹SNFECZHFMBUJO[ZNPHSBQIZBOEBDUJWJUZBT TBZT(FMBUJO[ZNPHSBQIZPGTFSVNGSFFI&.7&$DPOEJUJPOFENFEJB I TIPXFEFY QSFTTJPOPGMBUFOU..1 L%B BOEBL%BCBOEUIBUSFQSFTFOUT..1PS..1 'SPN UIF N3/" EBUB XF BTTVNF UIBU UIF L%B CBOE SFQSFTFOUT ..1 SBUIFS UIBO ..14UJNVMBUJPOXJUI.1."JOEVDFE..1 L%B QSPUFJOTZOUIFTJTBOEBD UJWBUJPOPG..1 L%B 'JHVSF" 5IFQSFTFODFPG.5..1XBTEFNPOTUSBUFECZ BDUJWJUZBTTBZ 'JHVSF# #PUI7&('"BOE5/'αFYQPTVSFEPVCMFEUIFBDUJWJUZPG.5 ..1 XIJMFQIPSCPMFTUFSDBVTFEBESBNBUJDJODSFBTFJO.5..1BDUJWJUZJOI&.7&$ 'JHVSF# 5IFQSFTFODFPG.5..1QSPUFJOXBTDPO¹SNFECZ8FTUFSOCMPUUJOH5IF QSPEVDUJPOPG.5..1XBTOPUBGGFDUFECZ5/'αBOEJODSFBTFETMJHIUMZBGUFS7&('" FYQPTVSF 'JHVSF$ .5..1XBTEFUFDUBCMFJOFOEPUIFMJBMDFMMTPGQSPMJGFSBUJWFIV NBOFOEPNFUSJBMUJTTVF BTXFMMBTJOFOEPNFUSJBMFQJUIFMJBMDFMMTBOENZPNFUSJBMDFMMT 'JHVSF%BOE& . B. C. 120. 7. 600. 2.5·106 pfu/mL 450 300. 100·106 pfu/mL. 150 0 control AdLacZ AdTIMP-1AdTIMP-3. MMP-9 activity (% of AdLacZ). TIMP-1 (% of control). 1.25·106 pfu/mL. active MT1-MMP (ng/mL). control 25 000. . 8. A. 40 000. 6 5 4 3 2 1 0. 90. 100-fold dilution. 25-fold dilution. 60. 30. 0 control. AdLacZ AdTIMP-1AdTIMP-3. AdLacZ. AdTIMP-1 AdTIMP-3. 'JHVSFI&.7&$PWFSFYQSFTTBDUJWF5*.1BOEBGUFSUSBOTEVDUJPO $POºVFOU I&.7&$ XFSF USBOTEVDFE XJUI ⋅ ⋅ BOE ⋅ QGVN- "E-BD; "E5*.1 PS "E5*.1 BT EFTDSJCFE JO UIF .FUIPET TFDUJPO "GUFS I UIF NFEJVN XBT SFNPWFE BOE UIF DFMMT XFSF JODVCBUFEGPSIXJUII&.7&$DVMUVSFNFEJVNBOEJODVCBUFEGPSIJO.TVQQMFNFOUFEXJUI )4" OHN-7&('"XJUIPSXJUIPVU1." . "5*.1MFWFMTXFSFEFUFSNJOFEJOUIFI&.7&$ DPOEJUJPOFE NFEJVN CZ &-*4" GPMMPXJOH UIF NBOVGBDUVSFST± EFTDSJQUJPOT # .5..1 BDUJWJUZ JO UIF MZTBUFT PG 1."TUJNVMBUFE USBOTEVDFE I&.7&$ XBT JOIJCJUFE CZ 5*.1 BOE OPU CZ 5*.1 .5..1 BDUJWJUZXBTBOBMZTFEBTJOEJDBUFEJOUIF.FUIPETTFDUJPO$..1BDUJWJUZXBTBTTBZFEVTJOHBCJPBDUJWJUZ BTTBZBTEFTDSJCFEJOUIF.BUFSJBMBOE.FUIPET5IFBCJMJUZPG5*.1BOE5*.1UPJOIJCJU..1BDUJWJUZ XBTNFBTVSFEJO CMBDLCBST BOEGPME HSBZCBST EJMVUJPOTPGUIFDPOEJUJPOFENFEJBPGI&.7&$ PWFSFYQSFTTJOH5*.1PS5*.1.

(14) .5..1JOFOEPNFUSJBMBOHJPHFOFTJTJOWJUSP. A. AdLacZ + BB94. AdTIMP-1. AdTIMP-3. B. 100 75. . . . 50. AdLacZ+ BB94. AdTIMP-1. 0. AdTIMP-3. . 25. AdLacZ. mean tube length (% of control). AdLacZ. 'JHVSF#PUI5*.1BOE5*.1JOIJCJUDBQJMMBSZMJLFUVCFGPSNBUJPOCZI&.7&$ I&.7&$ XFSF USBOTEVDFE XJUI ⋅ QGVN- "E-BD; "E5*.1 BOE "E5*.1 BOE XFSF DVMUVSFE PO UPQPGBUISFFEJNFOTJPOBM¹CSJONBUSJYPSB¹CSJODPMMBHFONBUSJYBOETUJNVMBUFEXJUI7&('" OHN- XJUIPSXJUIPVU## μHN- "1IBTFDPOUSBTUNJDSPHSBQITBGUFSEBZTPGDVMUVSJOHTIPXJOH UVCF GPSNBUJPO JO UIF ¹CSJO NBUSJY #BS μN # .FBO UVCF MFOHUI XBT NFBTVSFE BOE FYQSFTTFE BT B QFSDFOUBHF PG UIF UVCF GPSNBUJPO CZ UIF "E-BD;USBOTEVDFE DFMMT ± 4&.SBOHF PG ¹CSJO NBUSJY CMBDLCBST BOE ¹CSJODPMMBHFONBUSJY TUSJQFECBST JOEFQFOEFOUFYQFSJNFOUTQFSGPSNFEJOEVQMJDBUF XFMMT5IFNFBOUVCFMFOHUIPGUIF"E-BD;USBOTEVDFEI&.7&$XBT±NNDNPOUIF¹CSJODPMMBHFO NBUSJY∗1WT-BD;USBOTEVDFEDFMMT. .

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(16) .5..1JOFOEPNFUSJBMBOHJPHFOFTJTJOWJUSP. Mean tube length (mm/cm2 ). UJPOBMBOEBDUJWF UIFJSFGGFDUPO.5..1BOE..1BDUJWJUZXBTBOBMZTFE*ODPO USBTUUPDFMMFYUSBDUTPG"E-BD;PS"E5*.1USBOTEVDFEI&.7&$ JOXIJDI.5..1 SFNBJOFEBDUJWF .5..1BDUJWJUZXBTDPNQMFUFMZJOIJCJUFEJODFMMFYUSBDUTPG"E5*.1 USBOTEVDFEI&.7&$ 'JHVSF # "E5*.1 BOE "E5*.1USBOTEVDFE I&.7&$ JO IJCJUFEFYPHFOPVTBDUJWF..1DPNQBSBCMZ 'JHVSF$ 1SFWJPVTTUVEJFTPO)67&$BOEI'.7&$IBWFTIPXOUIBU5*.1XBTBNPSFQPUFOU JOIJCJUPSPGDBQJMMBSZUVCFGPSNBUJPOUIBO5*.1<-BºFVSFUBM $PMMFOFUBM "OBOE"QUFFUBM >6OFYQFDUFEMZ JOI&.7&$CPUI5*.1BOE5*.1PWFSFYQSFT TJPOJOIJCJUFE7&('"JOEVDFEUVCFGPSNBUJPO UPBOFYUFOUTJNJMBSBT## 'JHVSF" BOE # 5IJT XBT GPVOE CPUI JO ¹CSJO BOE JO ¹CSJODPMMBHFO NBUSJDFT 'JHVSF # /P BQQBSFOUDFMMEFBUIPSNPSQIPMPHJDBMDIBOHFTXFSFPCTFSWFEFJUIFSJOUIF"E5*.1PS "E5*.1USBOTEVDFEI&.7&$. 200 175. . 150 125. . 100 75 50 25 0. control. -. FITC. MT3. BB94. VEGF-A 'JHVSF*OIJCJUJPOPG.5..1SFEVDFTUVCFGPSNBUJPOCZI&.7&$ I&.7&$XFSFDVMUVSFEPOUPQPGBUISFFEJNFOTJPOBM¹CSJONBUSJYJO.TVQQMFNFOUFEXJUI)4BOE /#$4$FMMTXFSFDVMUVSFEVOEFSDPOUSPMDPOEJUJPOTJOUIFQSFTFODFPGOHNM7&('PSTUJNVMBUFE XJUI7&('" OHN- 7&('" OHN- BOEBOUJ'*5$*H( μHNM 7&('" OHN- BOEBOUJ .5..1 *H( α.5 μHNM PS 7&('" OHN- BOE ## μHN- .FBO UVCF MFOHUI XBT NFBTVSFEBOEFYQSFTTFEBTUIFNFBOUVCFMFOHUI±4&.PGFYQFSJNFOUTQFSGPSNFEJOEVQMJDBUFXFMMT∗ 1WT7&('TUJNVMBUFEI&.7&$1WT7&('BOUJ'*5$*H(USFBUFEI&.7&$. .

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(20)

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