The handle http://hdl.handle.net/1887/19165 holds various files of this Leiden University dissertation.
Author: Oosterhuis, Koen
Title: Preclinical development of DNA vaccine candidates for the treatment of HPV16 induced malignancies
Issue Date: 2012-06-27
Preclinical develoPment of
dna vaccine candidates for the treatment of hPv16 induced malignancies
Koen Oosterhuis
Preclinical develoPment of
dna vaccine candidates for the treatment of hPv16 induced malignancies
Proefschrift
ter verkrijging van
de graad van Doctor aan de Universiteit Leiden, op gezag van Rector Magnificus prof. mr. P.F. van der Heijden,
volgens besluit van het College voor Promoties te verdedigen op woensdag 27 juni 2012
klokke 16.15 uur
door
Koen Oosterhuis
geboren te Breukelen in 1980
Overige leden: Prof. Dr. S.H. van der Burgh Prof. Dr. G. Kenter (NKI-AVL) Prof. Dr. C.A.H.H. T. Daemen (UMCG) Dr. R.D.M. Steenbergen (VUmc)
The research described in this thesis was performed at the Department of Immunology at the Netherlands Cancer institute (NKI-AVL) and was financially supported by the Netherlands organization for health research en development (ZonMw) grant 432-00-001.
The printing of this thesis was financially supported by the Netherlands Cancer Institute.
Voor mijn ouders, voor Annelies, Eline & Merel
“How many roads must a man walk down, before you call him a man?”
Bob Dylan
table of contents
Chapter 1 General Introduction and outline 9
Chapter 2 DNA Vaccines and Intradermal Vaccination by DNA Tattooing
Curr. Top. Microbiol. Immunol.: 351, 221-50 (2012) 17
Chapter 3 Preclinical development of highly effective and safe DNA vaccines directed against HPV16 E6 and E7
Int. J. Cancer: 129, 397–406 (2011) 43
Chapter 4 Preclinical safety evaluation of DNA vaccines encoding modified HPV16 E6 and E7
Accepted for publication in Vaccine 63
Chapter 5 Rational design of DNA vaccines for the induction of HPV16 E6 and E7
specific cytotoxic T cell responses 83
Submitted for publication
Chapter 6 Shielding the cationic charge of nanoparticle-formulated dermal DNA vaccines is essential for antigen expression and immunogenicity
J. Control. Release: 141(2), 234-40 (2010) 105
Chapter 7 Summarizing discussion and future outlook 123
Annex Nederlandse Samenvatting 136
Curiculum Vitae 140
List of Publications 141
