Cover Page
The handle http://hdl.handle.net/1887/90130 holds various files of this Leiden University
dissertation.
Author: Witting, K.F.
Exploring the Ub/Ubl
Landscape with
Activity-based Probes
The research described in this thesis was initially performed in the Department of Cell Biology at the Netherlands Cancer Institute in Amsterdam, The Netherlands, and continued at the Department of Chemical Immunology and the Department of Cell and Chemical Biology at Leiden University Medical Center in Leiden, The Netherlands. The work was financially supported by a Marie Curie ITN Fellowship and Oncode Institute.
ISBN: 978-94-6402-188-2 Author: K.F. Witting
Cover Illustration: “The flow” by E.F. Witting
Complete design support: Ilse Modder | www.ilsemodder.nl
Financial Support: The Netherlands Cancer Institute and Leiden University Printed by: Gildeprint B.V. | www.gildeprint.nl
Copyright © 2020 by K.F. Witting. All rights reserved. No part of this publication may be reproduced or transmitted in any form or by any means without prior written permission of the author, or where appropriate, of the publisher of the articles.
Exploring the Ub/Ubl
Landscape with
Activity-based Probes
Proefschrift
ter verkrijging van
de graad van Doctor aan de Universiteit Leiden, op gezag van Rector Magnificus prof.mr. C.J.J.M. Stolker, volgens besluit van het College voor Promoties te verdedigen
op woensdag 20 mei 2020 klokke 11:15 uur
door
Katharina Felicitas Witting
Promotores
Prof. dr. H. Ovaa Prof. dr. J.J.C. Neefjes Dr. M.P.C. Mulder
Leden promotiecommissie
Prof. dr. F.G. Förster – Universiteit Utrecht Prof. dr. M.M. Maurice – Universiteit Utrecht Prof. dr. E.A. Reits—Academisch Medisch Centrum Prof. dr. P. ten Dijke
Prof. dr. R.C. Hoeben
Table of Contents
Chapter 1 | Introduction
Chapter 2 | Covalently capturing E2-E3 enzymes during Ubiquitin transfer with a genetically encoded 2,3-diaminopro-pionic acid active site
Chapter 3 | A cascading activity-based probe sequentially targets E1-E2-E3 Ubiquitin enzymes
Chapter 4 | Total chemical synthesis of SUMO and SUMO-based
probes for profiling the activity of SUMO-specific proteases
Chapter 5 | Generation of the UFM1 toolkit for profiling UFM1-
specific proteases and ligases
Chapter 6 | RPL26-UFMylation promotes interaction with SRP-
receptor during protein translocation
