PIP2 as local second messenger: a critical re-evaluation
Rheenen, Jacobus Emiel van
Citation
Rheenen, J. E. van. (2006, January 11). PIP2 as local second messenger: a critical re-evaluation. Retrieved from https://hdl.handle.net/1887/4337
Version: Corrected Publisher’s Version
License: Licence agreement concerning inclusion of doctoral thesis in theInstitutional Repository of the University of Leiden Downloaded from: https://hdl.handle.net/1887/4337
PIP
2
as
l
ocal
second
messenger:
a
cri
ti
cal
re-eval
uati
on
PIP2 as local second messenger:
a critical re-evaluation
PROEFSCHRIFT
t
er verkri
j
gi
ng van
de graad van Doct
or aan de Uni
versi
t
ei
t
Lei
den,
op gezag van de Rect
or M agni
fi
cus Dr.
D.
D.
Brei
mer,
hoogl
eraar i
n de facul
t
ei
t
der W i
skunde en
Nat
uurwet
enschappen en di
e der Geneeskunde,
vol
gens besl
ui
t
van het
Col
l
ege voor Promot
i
es
t
e verdedi
gen op W oensdag 11 j
anuari
2006
kl
okke 15.
15 uur
door
Jacobus Emi
el
van Rheenen
geboren t
e Ut
recht
Promotiecommissie
Promotor: Prof. Dr. J.J. Neefjes
Co-Promotor: Dr. K. Jalink (Nederlands Kanker Instituut, Amsterdam)
Referent: Prof. Dr. T. Schmidt
Overige leden: Prof. Dr. W.H. Moolenaar
Prof. Dr. P.J. Peters (Vrije Universiteit, Amsterdam) Dr. N. Divecha (Nederlands Kanker Instituut, Amsterdam) Dr. E.H. Danen (Nederlands Kanker Instituut, Amsterdam)
Supported by: The Netherlands Cancer Institute and The Netherlands Organization for Scientific Research (NWO)
Contents
Abbreviations
Chapter 1 Introduction
Chapter 2 PtdIns(4,5)P2 depletion is essential for stress-induced
apoptosis submitted
Chapter 3 Agonist-induced PIP2 hydrolysis inhibits cortical actin dy-
namics: Regulation at a global but not at a micrometer scale
Molecular biology of the Cell
Chapter 4 PIP2 signaling in lipid domains: a critical re-evaluation
EMBO Journal
Chapter 5 Correcting confocal acquisition to optimize imaging of fluorescence resonance energy transfer by sensitized
Abbreviations
ABP actin binding protein
CD methyl-E-cyclodextrin
CCD charge-coupled-device CCD cytochalasin D
CFP cyan fluorescent protein
DAG diacylglycerol
EM electron microscopy
ET endothelin
ETBr endothelin B receptor
FLIM fluorescence lifetime imaging FLIP fluorescence loss in photobleaching
FRAP fluorescence recovery after photobleaching FRET fluorescence resonance energy transfer
GFP green fluorescent protein
GPCR G protein-coupled receptor NKA neurokinin A
NK2r neurokinin A receptor
PH pleckstrin homology
PIP2 phosphatidylinositol (4,5) biphosphate
PIP3 phosphatidylinositol (3,4,5) triphosphate
PKC protein kinase C
PLC phospholipase C
PMT photomultiplier tubes
PSF pointspread function
RAP Reconstructed Axial PSF
RFP red fluorescent protein
ROI region of interest
YFP yellow fluorescent protein
