Cover Page
The following handle holds various files of this Leiden University dissertation:
http://hdl.handle.net/1887/59474
Author: Panagioti, E.
Title: Targeting and exploiting cytomegalovirus for vaccine development
Issue Date: 2017-12-05
TARGETING AND EXPLOITING CYTOMEGALOVIRUS FOR VACCINE DEVELOPMENT
Proefschrift
ter verkrijging van
de graad van Doctor aan de Universiteit Leiden, op gezag van Rector Magnificus prof. mr. C.J.J.M. Stolker,
volgens besluit van het college voor Promoties te verdedigen op
dinsdag 5 december 2017 klokke 15:00 uur
door
Eleni Panagioti geboren te Athene in 1988
The research described in this thesis was performed at the Departments of Medical Oncology and Immunohematology and Blood Transfusion of the Leiden University Medical Center, Leiden, The Netherlands and was funded by the EU under the FP7 Marie Curie Action VacTrain (Grant number: 316655). Financial support for the publication of this thesis was provided by the Leiden University Medical Center.
Cover Design: Eleni Panagioti & Botsas Design Studio. Inspired by Wassily Kandinsky’s colour studies and squares with concentric circles.
Layout & Printing: Off Page, Amsterdam
ISBN: 978-94-6182-855-2
Copyright © by 2017 E. Panagioti. All rights reserved. No part of this thesis may be reproduced in any form or by any means without permission of the author.
PROMOTOR
Prof. dr. S. H. van der Burg
CO-PROMOTOR
Dr. R. Arens
LEDEN PROMOTIECOMMISSIE:
Dr. M. G. H. Betjes (Erasmus University Medical Center, Rotterdam) Prof. dr. A. Geluk
Prof. dr. E.J.H.J. Wiertz (University Medical Center Utrecht, Utrecht) Prof. dr. B.A. M. van der Zeijst
TABLE OF CONTENTS
Chapter 1 General introduction 7
Chapter 2 The breadth of synthetic long peptide vaccine-induced CD8+ T cell 29 responses determines the efficacy against mouse cytomegalovirus infection Chapter 3 Enforced OX40 stimulation empowers booster vaccines to 59
induce effective CD4+ and CD8+ T cell responses against mouse cytomegalovirus infection
Appendix to chapter 3 91
Chapter 4 The spontaneous response to Human Cytomegalovirus immediate early 99 protein 2 is focused on 5 highly immunogenic regions and predominantly comprises polyfunctional type 1 CD4+ T cells
Chapter 5 The level of pre-existing immunity determines the efficacy of 127 MCMV-based vaccine vectors
Chapter 6 Requirements for effective T cell-inducing vaccines against 147 chronic viral infections
Chapter 7 General Discussion 169
Chapter 8 Appendix 183
English summary 185
Nederlandse samenvatting 187
Abbreviations 189
Curriculum vitae 190
List of publications 191
Aknowledgements 192
