Cover Page
The handle http://hdl.handle.net/1887/20472 holds various files of this Leiden University dissertation.
Author: Kim, Yeung-Hyen
Title: Mechanistic studies on human minor histocompatibility antigens in graft-versus- host disease
Issue Date: 2013-01-30
Mechanistic studies on human minor Histocompatibility antigens
in graft-versus-host disease
Yeung-Hyen Kim
Mechanistic studies on human minor Histocompatibility antigens
in graft-versus-host disease
Proefschrift
ter verkrijging van
de graad van Doctor aan de Universiteit Leiden,
op gezag van de Rector Magnificus Prof. mr
.P.F. van der Heijden volgens besluit van het College voor Promoties
te verdedigen op Woensdag 30 Januari 2013 klokke 16:15 uur
door
Yeung-Hyen Kim
geboren te Cheongdo, Zuid Korea in 1977
Promotiecommissie:
© Copyright by Yeung-Hyen Kim, 2013
ISBN: 978-90-6464-629-4
The research described in this thesis was conducted at the department of Immunohematology and Blood Transfusion at the Leiden University Medical Center and was financially supported by Dutch Cancer Society (KWF kanker bestrijding).
Financial support for the publication of this thesis was provided by the Dutch Cancer Society (KWF kanker bestrijding).
Cover picture: Detection of antigen-specific T cells in cryopreserved human skin tissues.
Designed by GVO drukkers & vormgevers B.V. | Ponsen & Looijen
Printed by: GVO drukkers & vormgevers B.V. | Ponsen & Looijen Promotor: Prof. Dr. E.A.J.M. Goulmy
Copromotor: Dr. E. Spierings Universiteit Utrecht
Overige leden: Prof. Dr. F.H.J. Claas Prof. Dr. A. Brand
Dr.T. Mutis Universiteit Utrecht
“For a research worker, the unforgotten moments of his life are those rare ones, which come after years of plodding work, when the veil over nature’s secret seem suddenly to lift and when what was dark and chaotic appears in a clear and beautiful light and pattern.”
- Gerty T. Cori -
For my family
Contents
Chapter 1 General introduction 9
Chapter 2 In situ detection of antigen-specific T cells 37
In situ visualization of antigen-specific T cells in cryopreserved human tissues.
J Immunol Methods. 2006 Mar 20;310(1-2):78-85
Chapter 3 HY T cells in skin GVHD 53
In situ detection of HY-specific T cells in acute graft-versus-host disease- affected male skin after sex-mismatched stem cell transplantation.
Biol Blood Marrow Transplant. 2012 Mar;18(3):381-7
Chapter 4 HA-1 T cells in skin GVHD 71
Exogenous addition of minor H antigen HA-1+ dendritic cells to skin tissues ex vivo causes infiltration and activation of HA-1-specific cytotoxic T cells.
Biol Blood Marrow Transplant. 2011 Jan;17(1):69-77
Chapter 5 Minor H antigens in GVHD and GVL 93 Multi-center analyses demonstrate significant clinical effects of minor
Histocompatibility antigens on GVHD and GVL after HLA matched related and unrelated stem-cell transplant.
Submitted for publication
Chapter 6 Summary and General discussion 115
Chapter 7 Dutch summary 129
Abbreviations 137
List of publications 141
Curriculum Vitae 145
Acknowledgements 149
