Pathogen inactivation in cellular blood products by photodynamic treatment Trannoy, L.L.

(1)

Pathogen inactivation in cellular blood products by photodynamic treatment

Trannoy, L.L.

Citation

Trannoy, L. L. (2010, May 12). Pathogen inactivation in cellular blood products by photodynamic treatment. Retrieved from

https://hdl.handle.net/1887/15371

Version: Corrected Publisher’s Version

License: Licence agreement concerning inclusion of doctoral thesis in the Institutional Repository of the University of Leiden

Downloaded from: https://hdl.handle.net/1887/15371

Note: To cite this publication please use the final published version (if

applicable).

(2)

Pathogen inactivation in cellular blood products by

photodynamic treatment

PROEFSCHRIFT

ter verkrijging van

de graad van Doctor aan de Universiteit Leiden, op gezag van Rector Magnificus prof.mr. P.F. van der Heijden,

volgens besluit van het College voor Promoties te verdedigen op woensdag 12 mei 2010

klokke 11:15 uur

door

Laurence Liliane Trannoy geboren te Soissons (Frankrijk)

in 1973

(3)

Promotiecommissie

Promotor: Prof.dr. A. Brand

Co-promotor: Dr. J.W.M. Lagerberg (Sanquin Research, Amsterdam) Overige leden: Prof.dr. F.H.J. Claas

Prof.dr. F. Koning

Prof.dr. H.J. Zaaijer (Amsterdam Medisch Centrum)

The research described in this thesis was conducted at the Sanquin Blood Bank Southwest Region, at the department of Immunohematology and Blood Transfusion and at the department of Molecular and Cellular Biology of the Leiden University Medical Center, Leiden. The studies were financed by ZonNW (grant nr. 28-24140).

ISBN: 978-90-9025274-2

Layout and cover design: L. Trannoy Printed by: Ipskamp Drukkers B.V.

Publication of this thesis was financially supported by Sanquin Blood Bank,

Buchem B.V., Beckman Coulter B.V., and Greiner Bio-One B.V.

(4)

4

ABBREVIATIONS

1O2 = singlet oxygen

AAMφ = alternatively activated macrophages AlPcS4 = Tetrasulfonated aluminum

phthalocyanine APC = Antigen presenting cells

ATCC = American Type Culture Collection ATP = Adenosine triphosphate

BFU-E = Burst forming unit of erythrocytes BM = Bone marrow

BVDV = Bovine viral diarrhoea virus CB = Cord blood

cb = Cytochalasine B CBSC = Cord blood stem cells CFU = Colony forming unit

CFU-GEM = Mixed CFU of granulocytes, monocytes and erythrocytes

CFU-GM = CFU of granulocytes and monocytes

CFU-Mk = CFU of megakaryocytes CPV = Canine parvovirus

DAT = Direct gel agglutination test DIP = Dipyridamole

DMMB = Dimethyl-methylene blue G- = gram negative

G+ = gram positive GSH = reduced glutathione GvHD = Graft-versus-host disease Hb = Hemoglobin

Hct = Hematocrit HBV = Hepatitis B virus HCV = Hepatitis C virus HES = Hydroxy-ethyl starch

HIV = Human immunodeficiency virus HLA = Human leukocytes antigens HPC = Hematopoietic progenitor cells HTLV = Human T-lymphoma virus IAGT = Indirect Antiglobulin Test LPS = Lipopolysaccharide MB = Methylene blue MNC = Mononuclear cells

MLR = Mixed lymphocytes reaction NaP = sodium phosphate buffer NAT = Nucleic acid test

NHFTR = non-hemolytic febrile transfusion reactions

PBS = Phosphate-buffered saline PC = Platelet concentrate PDT = Photodynamic treatment PHA = Phytohemagluttin PPV = Porcine parvovirus PRV = Pseudorabies virus RBC = Red blood cells

RBCC = Red blood cell concentrate ROS = Reactive oxygen species SD = solvent/detergent

SAG-M = Saline Adenine Glucose Mannitol T50 = half lifespan

TCID₅₀ = Tissue Culture Infectious Dose (50%)

Tri-P(4) = mono-phenyl-tri-(N-methyl-4- pyridyl)-porphyrin chloride

VSV = Vesicular stomatitis virus WBC = White blood cells WNV = West-Nile virus

(5)

5

Chapter I Introduction

Chapter II Relation between K

⁺

leakage and damage to Band 3 in photodynamically treated red cells.

Chapter III Selective protection of red blood cells against photo- dynamic damage by the Band 3 ligand dipyridamole.

Chapter IV Positively charged porphyrins: a new series of photo- sensitizers for pathogen inactivation in red blood cell concentrates.

Chapter V Differential sensitivities of pathogens in red blood cell concentrates to Tri-P(4)-photoinactivation.

Chapter VI Photodynamic treatment with Tri-P(4) for pathogen inactivation in cord blood stem cell products.

Chapter VII Impact of photodynamic treatment with porphyrin Tri-P(4) on the immunomodulatory capacity of white blood cells- containing red blood cell products.