University of Groningen
Applying lipidomics strategies to study lipid metabolic diseases
Zhang, Wenxuan
DOI:
10.33612/diss.169407826
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Publication date: 2021
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Citation for published version (APA):
Zhang, W. (2021). Applying lipidomics strategies to study lipid metabolic diseases. University of Groningen. https://doi.org/10.33612/diss.169407826
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Applying lipidomics strategies to
study lipid metabolic diseases
The research described in this thesis was carried out at the Department of Pediatrics, Center for Liver, Digestive and Metabolic Disease, University Medical Center Groningen, Groningen, the Netherlands.
The studies described in this thesis were financially supported by the SysMed Grant from the University Medical Center Groningen.
The printing of this dissertation was financially supported by: University of Groningen (RuG)
University Medical Center Groningen (UMCG)
Groningen University Institute of Drug Exploration (GUIDE) Cover design: Guus Gijben | proefschrift-aio.nl
Book layout: Guus Gijben | proefschrift-aio.nl Printed by: proefschrift-aio.nl
© Wenxuan Zhang, 2021
All rights reserved. No part of this doctoral thesis may be reproduced, stored in a retrieval system or transmitted in any form or by any means without permission of the author.
Applying lipidomics strategies to study
lipid metabolic diseases
PhD thesis
to obtain the degree of PhD at the University of Groningen
on the authority of the Rector Magnificus Prof. C. Wijmenga
and in accordance with the decision by the College of Deans. This thesis will be defended in public on
Monday 10 May 2021 at 12.45 hours
by
Wenxuan Zhang
born on 22 September 1990 in Xi’an, China
Supervisors
Prof. F. Kuipers Prof. R.P.H. Bischoff Prof. D.J. Reijngoud
Assessment Committee
Prof. F.J. van Spronsen Prof. J. Heeren
Table of Content
Introduction - Chapter 1 9
Part I Evaluation of lipid extraction systems
Chapter 2 Omics technology: lipidomics and its pitfalls during the preanalytical stage
27
Chapter 3 One- vs two-phase extraction: re-evaluation of sample preparation procedures for untargeted lipidomics in plasma samples
55
Part II Application of lipidomics to disease-related studies
Chapter 4 The plasma lipidome of individuals with extreme levels of LDL-c
79
Chapter 5 The role of hepatic fatty-acid oxidation during cold stress: remodeling of whole body energy and lipid metabolism in Acadm-knockout mice.
105
Chapter 6 Low LRH-1 expression in mice promotes development of Western-type diet-induced steatohepatitis and liver fibrosis
Multi-omics integration reveals a critical role of LRH-1 in adequate adaptation of hepatic lipid metabolism to nutrient overload
Part III Discussion
Chapter 7 General discussion 171
Appendix Summary 186 Nederlandse samenvatting 190 中文摘要 194 Acknowledgement 196 Curriculum vitae 200 List of Publications 202
