Cover Page
The handle http://hdl.handle.net/1887/28275 holds various files of this Leiden University dissertation.
Author: O’Flynn, Joseph
Title: Properdin-dependent activation and control of immune-homeostasis and autoimmunity
Issue Date: 2014-08-27
Properdin-dependent activation and control of immune-homeostasis
and autoimmunity
Joseph O’Flynn
Properdin-dependent activation and control of immune-homeostasis and autoimmunity
© Joseph O’Flynn 2014
All rights are reserved. No parts of this publication may be reproduced, stored, or transmitted in any form or by any means, without permission of the copyright owners.
ISBN: 978-90-5335-890-0 Layout: Joseph O’Flynn Printed by: Ridderprint.nl Published by: Ridderprint.nl
The research presented in this thesis was performed at the Department of Nephrology, Leiden University Medical Center, The Netherlands.
The research described in this thesis was supported by a Marie Curie TranSVIR fellowship (TranSVIR FP7-PEOPLE-ITN-2008 No.238756) funded by the European commission under the 7th framework programme.
Financial support by the Dutch Kidney Foundation, Astellas Pharma B.V, Hycult Biotech and TECO Medical for the publication of this thesis is gratefully acknowledged.
Properdin-dependent activation and control of immune-homeostasis
and autoimmunity
Proefschrift
ter verkrijging van
de graad van doctor aan de Universiteit Leiden, op gezag van de Rector Magnificus Prof.mr. C.J.J.M. Stolker,
volgens besluit van het College voor Promoties te verdedigen op 28 august 2014
klokke 15.00 uur
door
Joseph O’Flynn (Seosamh O’Floinn)
born in Galway, Republic of Ireland in 1987
Promotiecommissie
Promotores: Prof. Dr. C. van Kooten
Prof. Dr. M.R. Daha
Overige leden: Prof. Dr. A.J. Rees
Medical University of Vienna, Vienna, Austria Prof. Dr. P.S. Hiemstra
Dr. M.A. Seelen
University Medical Center Groningen, Groningen Dr. L.A. Trouw
Our greatest glory consists not in never falling, but in rising every time we fall.
Oliver Goldsmith
(10 November 1730 - 4 April 1774)
Contents
Chapter 1: General Introduction 9 Chapter 2: Nucleosomes and C1q bound to glomerular endothelial cells serve
as targets for autoantibodies and determine complement activation.
Mol Immunol 49 (2011) 75–83. 29
Chapter 3: Properdin binding independent of complement activation in an in-vivo model of anti-GBM disease.
In preparation. 49
Chapter 4: Purification and functional analysis of human properdin.
Methods Mol Biol. 2014;1100:161-7. 65 Chapter 5: Myeloperoxidase directs properdin-mediated complement
activation.
J Innate Immun 2014;6(4):417-25. 75 Chapter 6: CRP as a novel inhibitor of Properdin and complement activation.
In preparation. 91
Chapter 7: Properdin and factor H production by human dendritic cells is differentially regulated by IFNγ and has a functional role in the T cell stimulatory capacity of dendritic cells.
Submitted. 109
Chapter 8: General discussion 131 Chapter 9: Nederlandse samenvatting
Irish and English summary List of abbreviations, Curriculum vitae, Acknowledgements,
Bibliography 147
