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University of Groningen VPS13A: shining light on its localization and function Faber, Anna Irene Elizabeth

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University of Groningen

VPS13A: shining light on its localization and function

Faber, Anna Irene Elizabeth

IMPORTANT NOTE: You are advised to consult the publisher's version (publisher's PDF) if you wish to cite from

it. Please check the document version below.

Document Version

Publisher's PDF, also known as Version of record

Publication date:

2019

Link to publication in University of Groningen/UMCG research database

Citation for published version (APA):

Faber, A. I. E. (2019). VPS13A: shining light on its localization and function. Rijksuniversiteit Groningen.

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VPS13A: Shining Light On Its

Localization And Function

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The research described in this thesis was performed at the Department of Cell Biology of the University Medical Center Groningen, The Netherlands.

Printing of this thesis was financially supported by the University of Groningen, The Netherlands. ISBN: 978-94-034-1615-1 (printed version)

ISBN : 978-94-034-1614-4 (electronic version)

Copyright © 2019 A.I.E. Faber, Groningen, The Netherlands. All rights reserved. No part of this book may be reproduced or transmitted in any form or by any means without prior written permission of the author.

Cover and layout design by ThesisExpert.nl Printed by Gildeprint, Enschede, The Netherlands

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VPS13A: Shining light on its

localization and function

Proefschrift

ter verkrijging van de graad van doctor aan de

Rijksuniversiteit Groningen

op gezag van de

rector magnificus prof. dr. E. Sterken

en volgens besluit van het College voor Promoties.

De openbare verdediging zal plaatsvinden op

woensdag 15 mei 2019 om 14.30 uur

door

Anna Irene Elizabeth Faber

geboren op 22 december 1988

te Juigalpa, Nicaragua

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Promotor

Prof. dr. O.C.M. Sibon

Copromotor

Dr. B.N.G. Giepmans

Beoordelingscommissie

Prof. dr. L.W. Beukeboom

Prof. dr. A. Danek

Prof. dr. B. Brundel

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TABLE OF CONTENTS

Chapter 1

Introduction and aim of the thesis

Chapter 2

Modelling in miniature: using Drosophila melanogaster to study human

neurodegeneration

Chapter 3

Drosophila Vps13 is required for protein homeostasis in the brain

Chapter 4

CRISPR/Cas9 based genome editing of Drosophila for the generation of

Vps13 knockout mutant and Vps13-GFP flies

Chapter 5

Timely removal of nurse cell corpses requires cell-autonomous function

of

Vps13

Chapter 6

Human VPS13A is associated with multiple organelles and required for

lipid droplet homeostasis

Chapter 7

General discussion

Appendices

Nederlandse samenvatting

Acknowledgements

7

19

31

59

85

119

159

170

174

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