University of Groningen
Mechanism of the translocon
Taufik, Intan
DOI:
10.33612/diss.102814953
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Publication date: 2019
Link to publication in University of Groningen/UMCG research database
Citation for published version (APA):
Taufik, I. (2019). Mechanism of the translocon: events at the gate. University of Groningen. https://doi.org/10.33612/diss.102814953
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Mechanism of The Translocon:
Events at The Gate
Mechanism of The Translocon: Events at The Gate Intan Taufik
PhD thesis
University of Groningen
ISBN: 978-94-034-2226-8 (printed version) ISBN: 978-94-034-2225-1 (electronic version)
The research presented in the thesis was performed in the Molecular Microbiology research group, which is part of the Groningen Biomolecular Sciences and Biotechnology institute (GBB) of the Universtity of Groningen, the Netherlands.
Cover design and layout by: Firman Mustari Printed by: Ipskamp Printing
Copyright © 2019 by Intan Taufik
All rights reserved. No part of this thesis may be reproduced, stored in a retrieval system or transmitted in any form or by any means without the prior written permission of the author.
Mechanism of The Translocon:
Events at The Gate
PhD thesis
to obtain the degree of PhD at the University of Groningen
on the authority of the
Rector Magnificus Prof. Cisca Wijmenga and in accordance with
the decision by the College of Deans. This thesis will be defended in public on
Friday 6 December 2019 at 12.45 hours
by
Intan Taufik
born on 28 February 1975 in Bandung, Indonesia
Supervisor
Prof. A.J.M. Driessen Assessment Committee Prof. J.M. van Dijk
Prof. O.P. Kuipers Prof. W. Bitter
For my wife Enci,
daughter Bianglala &
son Azzam
Table of Contents
Chapter 1 The Working Translocon – Structure and Mechanism 9 Chapter 2 The Lateral Gate of SecYEG Opens to its Full Length 35
To Facilitate Protein Translocation
Chapter 3 Introduction of a Long Spanning Optical Switch into 51 The SecYEG Lateral Gate
Chapter 4 Monitoring the Activity of Single Translocons 63
Journal of Molecular Biology (2013) 425 (22): 4145-4153
Chapter 5 Dynamics of the Interaction between the Two Helix 79 Finger of SecA ATPase and SecYEG
References 93
Summary and Perspective 121
Samenvatting en Perspectief 127
Ringkasan dan Persepektif 133
Acknowledgements 139
