University of Groningen
Triggering pneumococcal competence
Slager, Jelle
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2019
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Slager, J. (2019). Triggering pneumococcal competence: Memoirs of an escape artist. Rijksuniversiteit
Groningen.
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Triggering pneumococcal competence
Memoirs of an escape artist
The research presented in this thesis was carried out in the group of Molecular Genetics of the Groningen Biomolecular Sciences and Biotechnology Institute (GBB), Faculty of Science and Engineering, University of Groningen, The Netherlands and the group of Systems and Synthetic Microbiology of the Department of Fundamental Microbiology (DMF), Faculty of Biology and Medicine, University of Lausanne, Switzerland.
Printing of this thesis was financially supported by the Groningen Graduate School of Science and the University of Groningen.
Printed by: Ridderprint BV, Ridderkerk, The Netherlands
Cover design: Majken Enequist
Copyright © 2019 Jelle Slager, Groningen, The Netherlands All rights reserved
ISBN (printed): 978-94-034-1316-7
Triggering pneumococcal
competence
Memoirs of an escape artist
Proefschrift
ter verkrijging van de graad van doctor aan de
Rijksuniversiteit Groningen
op gezag van de
rector magnificus prof. dr. E. Sterken
en volgens besluit van het College voor Promoties.
De openbare verdediging zal plaatsvinden op
vrijdag 22 februari 2019 om 16.15 uur
door
Jelle Slager
geboren op 19 november 1986
te Dongeradeel
Promotores
Prof. dr. J.-W. Veening
Prof. dr. O.P. Kuipers
Beoordelingscommissie
Prof. dr. L.W. Hamoen
Prof. dr. J. Kok
Contents
General introduction
Deep genome annotation of the opportunistic human pathogen Streptococcus pneumoniae D39
High-resolution analysis of the pneumococcal transcriptome under a wide range of infection-relevant conditions
Refining the pneumococcal competence regulon by RNA-sequencing
Antibiotic-induced replication stress triggers bacterial competence by increasing gene dosage near the origin
Antibiotic-induced cell chaining triggers pneumococcal competence by reshaping quorum sensing to autocrine-like signaling
Summary and general discussion
Wetenschappelijke samenvatting Samenvatting voor de leek Dankwoord - Acknowledgments Publication overview Chapter 1 Chapter 2 Chapter 3 Chapter 4 Chapter 5 Chapter 6 Chapter 7 9 25 63 95 129 151 177 193 205 217 227
