University of Groningen
Anatomy of the pneumococcal nucleoid
van Raaphorst, Renske
DOI:
10.33612/diss.127742005
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Publication date: 2020
Link to publication in University of Groningen/UMCG research database
Citation for published version (APA):
van Raaphorst, R. (2020). Anatomy of the pneumococcal nucleoid: Visualizing replication, chromosome segregation and chromosome condensation dynamics in Streptococcus pneumoniae. University of Groningen. https://doi.org/10.33612/diss.127742005
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Anatomy
of the
pneumococcal nucleoid
Visualizing replication, chromosome segregation and
chromosome condensation dynamics in
Streptococcus pneumoniae
The research described in this thesis was carried out at the department of Molecular Genetics (MolGen), Groningen Biochemistry and Biotechnology Institute (GBB), Faculty of Science and Engineering (FSE), University of Groningen, the Netherlands, and at the Veening Lab of the Department of Fundamental Microbiology (DMF), Faculty of Biology and Medicin (FBM), University of Lausanne, Switzerland.
Printing of this theisis was fi ncancially supported by the Groningen Graduate School of Science and the University of Groningen.
Print: Ridderprint | www.ridderprint.nl
Cover design: Renske van Raaphorst. Electron microscopy image from Veening lab collection, imaged by C. Gallay, S. Sanselicio et al., bioRxiv 2019.
Copyright © Renske van Raaphorst, Woluwe St-Lambert, Belgium All rights reserved
ISBN (print) : 978-94-034-2696-9 ISBN (digital) : 978-94-034-2697-6
Anatomy of the pneumococcal
nucleoid
Visualizing replication, chromosome segregation and
chromosome condensation dynamics in Streptococcus
pneumoniae
Proefschrift
ter verkrijging van de graad van doctor aan de Rijksuniversiteit Groningen
op gezag van de
rector magnificus prof. dr. C. Wijmenga en volgens besluit van het College voor Promoties.
De openbare verdediging zal plaatsvinden op vrijdag 26 juni 2020 om 16.15 uur
door
Renske van Raaphorst
geboren op 30 mei 1989 te Texel
Promotores
Prof. dr. Jan-Willem Veening
Prof. dr. Oscar P. Kuipers
Beoordelingscommissie
Prof. dr. Stephan Gruber
Prof. dr. Jan-Maarten van Dijl
Prof. dr. Marc Bramkamp
Contents
Chapter 1 Introduction 8
Chapter 2 Red Fluorescent Proteins for gene expres-sion and protein localization studies in
Streptococcus pneumoniae and efficient
transformation with DNA assembled via the Gibson Assembly method
24
Chapter 3 BactMAP: an R package for integrating, analyzing and visualizing bacterial microscopy data
44
Chapter 4 Chromosome segregation drives division
site selection in Streptococcus pneuminae 68 Chapter 5 Investigations into the condensation and
segregation dynamics of the pneumo- coccal nucleoid
114
Chapter 6 General discussion 142
Summary Scientific summary Wetenschappelijke samenvatting 154 156 166 Popular summary
Samenvatting voor het brede publiek
180 184
Tot slot
Acknowledgements/dankwoord About the author
Publications
188 190 195 197
