• No results found

University of Groningen Vitamin B12 Transport in Bacteria Rempel, Stephan

N/A
N/A
Protected

Academic year: 2021

Share "University of Groningen Vitamin B12 Transport in Bacteria Rempel, Stephan"

Copied!
9
0
0

Bezig met laden.... (Bekijk nu de volledige tekst)

Hele tekst

(1)

University of Groningen

Vitamin B12 Transport in Bacteria

Rempel, Stephan

IMPORTANT NOTE: You are advised to consult the publisher's version (publisher's PDF) if you wish to cite from it. Please check the document version below.

Document Version

Publisher's PDF, also known as Version of record

Publication date: 2019

Link to publication in University of Groningen/UMCG research database

Citation for published version (APA):

Rempel, S. (2019). Vitamin B12 Transport in Bacteria: A structural and biochemical study to identify new transport systems. University of Groningen.

Copyright

Other than for strictly personal use, it is not permitted to download or to forward/distribute the text or part of it without the consent of the author(s) and/or copyright holder(s), unless the work is under an open content license (like Creative Commons).

Take-down policy

If you believe that this document breaches copyright please contact us providing details, and we will remove access to the work immediately and investigate your claim.

Downloaded from the University of Groningen/UMCG research database (Pure): http://www.rug.nl/research/portal. For technical reasons the number of authors shown on this cover page is limited to 10 maximum.

(2)

Vitamin B12 Transport in Bacteria

A structural and biochemical study to identify new transport systems

(3)

Cover: The Power of Transport – engine room of the MS Cap San

Diego, one of the last seaworthy pre-container era general

cargo ships in the world. Cover design: Stephan Rempel

ISBN (print version): 978-94-034-1284-9 ISBN (online version): 978-94-034-1283-2

Printed by: Optima Grafische Communicatie B.V. – The Netherlands

The research described in this thesis was carried out in the Membrane Enzymology Group of the Groningen Biomolecular and Biotechnology (GBB) Institute of the University of Groningen, The Netherlands. The Netherlands Organization for Scientific Research (NWO), the European Research Council (ERC), and the European Molecular Biology Organization (EMBO) funded the research. The Stichting Stimulering Biochemie Nederland (SSBN-NVBMB) and the German Science Foundation (DFG) supported the project with travel grants.

© 2018 Stephan Rempel

All rights reserved. This book or any portion thereof may not be reproduced or used in any manner whatsoever without the express written permission of the author.

(4)

Vitamin B12 transport in bacteria

A structural and biochemical study to identify new transport systems

PhD thesis

to obtain the degree of PhD at the University of Groningen

on the authority of the Rector Magnificus Prof. E. Sterken

and in accordance with the decision by the College of Deans. This thesis will be defended in public on Friday 18th of January 2019 at 16:15 hours

by

Stephan Rempel

born on 24 June 1989 in Wertheim, Germany

(5)

Supervisors

Prof. D.J. Slotboom Prof. B. Poolman

Assessment Committee

Prof. S.J. Marrink Prof. A.J.M. Driessen Prof. M.A. Seeger

(6)

“Technology is a big destroyer of emotion and truth, […] it makes it easier and you can get home sooner; but it doesn’t make you a more creative person. That’s [what] we have to fight in any creative field:

ease of use.”

(7)
(8)

Table of Contents

Chapter 1

ECF-type ABC transporters

9

Chapter 2

Functional and structural characterization

of an ECF-type ABC transporter for

vitamin B12

55

Chapter 3

Cysteine-mediated decyanation of vitamin

B12 by the predicted membrane

transporter BtuM

83

Chapter 4

On the role of modifications for the

oligomeric state of the vitamin B12

transporter BtuM

119

Chapter 5

Summary and perspective on vitamin B12

transport

135

Addendum

Summaries in Dutch and German in

layman terms

List of publications and achievements

Acknowledgments

(9)

Referenties

GERELATEERDE DOCUMENTEN

Although the molecular identity of ECF-type ABC transporters remained elusive at the time, the results cumulated in the remarkably accurate description of the function

in 2009 (15) predicted that the energy coupling factor (ECF-) type ABC transporter ECF-CbrT might be a Cbl transporter (15). ECF-transporters are multi-subunit membrane complexes

Cbl-bound mutants H28A, Y85L, and R153A displayed the same spectral properties as the WT protein (Suppl. Figure 10a), and MS analysis showed that the binding of Cbl

Although the fusion to a fluorescent Ypet protein affects the activity of BtuM Td , the in vivo oligomeric state determination is of value, since in vivo experiments

The crystal structure of BtuMTd additionally revealed an unprecedented binding mode of vitamin B12 that is linked to chemical modification of the substrate prior

Ungefähr die Hälfte aller bekannten Bakterien benötigen einen Vitamin B12 Transporter, dennoch ist bisher nur ein Transporter für dieses Vitamin bekannt, den

Transport of each of these substrates depended on a specific, membrane- bound binding protein (now named S-component), and a shared component, for which different

Vitamin B12 Transport in Bacteria: A structural and biochemical study to identify new transport systems1. University