University of Groningen
On the mechanism of proton-coupled transport by the maltose permease of Saccharomyces cerevisiae
Henderson, Ryan
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Publication date: 2019
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Henderson, R. (2019). On the mechanism of proton-coupled transport by the maltose permease of Saccharomyces cerevisiae. University of Groningen.
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On the mechanism of proton-coupled
transport by the maltose permease of
Saccharomyces cerevisiae
-Cover: Artist's impression of the process of progressively uncoupling the transport of sugar from the transport of protons. Designed by Marina Go-mez Fuentes
ISBN (electronic): 978-94-034-1279-5 ISBN (print): 978-94-0340-1280-1
Printed by: Optima Grafische Communicatie B.V., Rotterdam
The work presented in this thesis was carried out in the Membrane Enzy-mology group of the Groningen Biomolecular Sciences and Biotechnology Institute (GBB) at the University of Groningen and was financially sup-ported by the BE-Basic R&D Program, which was granted a FES subsidy from the Dutch Ministry of Economic affairs, agriculture and innovation (EL&I). The research was also funded by a NWO TOP-PUNT (project number 13.006) grant.
Copyright © 2018 by Ryan Kenneth Henderson. All rights reserved. No parts of this thesis may be reproduced, stored in a retrieval system, or trans-mitted in any form or by any means, without the permission of the author.
On the mechanism of proton-coupled
transport by the maltose permease of
Saccharomyces cerevisiae
PhD thesis
to obtain the degree of PhD at the University of Groningen
on the authority of the Rector Magnificus Prof. E. Sterken
and in accordance with the decision by the College of Deans. This thesis will be defended in public on
Friday 11 January 2019 at 11.00 hours
by
Ryan Kenneth Henderson
born on Monday 30 September 1991 in Miami, Florida, United States of America
Supervisors
Prof. B. Poolman Prof. D.J. Slotboom
Assessment Committee
Prof. A.J.M. Driessen Prof. D.B. Janssen Prof. A.M.A. van Maris
Chapter 1 Chapter 2 Chapter 3 Chapter 4 Chapter 5 Chapter 6
Table of Contents
Energetics and regulation of secondary active transport: insight from structures and translocation kinetics
Proton-solute coupling mechanism of the maltose transporter from Saccharomyces
cerevisiae
Second-site suppressors of uncoupled mutants provide insight into the energy coupling mechanism of Mal11
Characterization of maltose-binding residues in the central cavity of Mal11
Expression and purification of the Mal11 α-glucoside transporter from Saccharomyces
cerevisiae
Conclusions and Perspectives References Summary Nederlandse Samenvatting Acknowledgements Biography 7 19 41 57 73 91 99 111 115 119 123
