University of Groningen
Exploring (per)oxidases as biocatalysts for the synthesis of valuable aromatic compounds
Habib, Mohamed H M
DOI:
10.33612/diss.109693881
IMPORTANT NOTE: You are advised to consult the publisher's version (publisher's PDF) if you wish to cite from it. Please check the document version below.
Document Version
Publisher's PDF, also known as Version of record
Publication date: 2020
Link to publication in University of Groningen/UMCG research database
Citation for published version (APA):
Habib, M. H. M. (2020). Exploring (per)oxidases as biocatalysts for the synthesis of valuable aromatic compounds. University of Groningen. https://doi.org/10.33612/diss.109693881
Copyright
Other than for strictly personal use, it is not permitted to download or to forward/distribute the text or part of it without the consent of the author(s) and/or copyright holder(s), unless the work is under an open content license (like Creative Commons).
Take-down policy
If you believe that this document breaches copyright please contact us providing details, and we will remove access to the work immediately and investigate your claim.
Downloaded from the University of Groningen/UMCG research database (Pure): http://www.rug.nl/research/portal. For technical reasons the number of authors shown on this cover page is limited to 10 maximum.
Exploring (per)oxidases as
biocatalysts for the
synthesis of valuable
aromatic compounds
The research described in this thesis was carried out in the department of Molecular Enzymology of the Groningen Biomolecular Sciences and Biotechnology Institute according to the requirements of the Graduate School of Science, Faculty of Mathematics and Natural Sciences, University of Groningen, The Netherlands. Mohamed Habib received funding from the Cultural Affairs and Missions Sector, Ministry of Higher Education in Egypt.
Cover design: Mohamed Habib and Nina Lindhout Printed by:
ISBN: ISBN:
Copyright © Mohamed Habib 2020, Groningen, The Netherlands.
All rights reserved. No part of this thesis may be reproduced, stored in a retrieval system, or transmitted in any form or by any means without prior permission of the author.
Exploring (per)oxidases as
biocatalysts for the
synthesis of valuable
aromatic compounds
PhD thesis
to obtain the degree of PhD at the
University of Groningen
on the authority of the
Rector Magnificus Prof. C. Wijmenga
and in accordance with
the decision by the College of Deans.
This thesis will be defended in public on
Monday, January 20, 2020 at 12:45 pm hours
by
Mohamed Habib
born on 8 April 1987 in Riyadh, Saudi Arabia
Printed by: Ipskamp Drukkers B.V. ISBN (printed version): 978-94-034-2334-0 ISBN (electronic version): 978-94-034-2335-7
The research described in this thesis was carried out in the department of Molecular Enzymology of the Groningen Biomolecular Sciences and Biotechnology Institute according to the requirements of the Graduate School of Science, Faculty of Mathematics and Natural Sciences, University of Groningen, The Netherlands. Mohamed Habib received funding from the Cultural Affairs and Missions Sector, Ministry of Higher Education in Egypt.
Cover design: Mohamed Habib and Nina Lindhout Printed by:
ISBN: ISBN:
Copyright © Mohamed Habib 2020, Groningen, The Netherlands.
All rights reserved. No part of this thesis may be reproduced, stored in a retrieval system, or transmitted in any form or by any means without prior permission of the author.
Exploring (per)oxidases as
biocatalysts for the
synthesis of valuable
aromatic compounds
PhD thesis
to obtain the degree of PhD at the
University of Groningen
on the authority of the
Rector Magnificus Prof. C. Wijmenga
and in accordance with
the decision by the College of Deans.
This thesis will be defended in public on
Monday, January 20, 2020 at 12:45 pm hours
by
Mohamed Habib
born on 8 April 1987 in Riyadh, Saudi Arabia
Supervisors
Prof. M.W. Fraaije Prof. D.B. Janssen
Assessment Committee
Prof. W.J.H. van Berkel Prof. G. Maglia
Prof. F. Hollmann
Table of Contents
Chapter I General introduction: Oxidases and peroxidases and their roles in biocatalysis 7
Chapter II Bacterial enzymes involved in lignin degradation 26
Chapter III A biocatalytic one‐pot approach for the preparation of lignin oligomers using an oxidase/peroxidase cascade enzyme system 51
Chapter IV The biocatalytic synthesis of syringaresinol from 2,6-dimethoxy-4-allylphenol in one-pot using a tailored oxidase/peroxidase system 70
Chapter V Characterization of a new DyP-peroxidase from the alkaliphilic cellulomonad, Cellulomonas bogoriensis 82
Chapter VI Expression and characterization of a resurrected DyP-type peroxidase 100
Summary, Conclusion and Future Outlook 120
Nederlandse Samenvatting 126
Curriculum vitae 131
Acknowledgements 132
Supervisors
Prof. M.W. Fraaije Prof. D.B. Janssen
Assessment Committee
Prof. W.J.H. van Berkel Prof. G. Maglia
Prof. F. Hollmann
Table of Contents
Chapter I General introduction: Oxidases and peroxidases and their roles in biocatalysis 7
Chapter II Bacterial enzymes involved in lignin degradation 26
Chapter III A biocatalytic one‐pot approach for the preparation of lignin oligomers using an oxidase/peroxidase cascade enzyme system 51
Chapter IV The biocatalytic synthesis of syringaresinol from 2,6-dimethoxy-4-allylphenol in one-pot using a tailored oxidase/peroxidase system 70
Chapter V Characterization of a new DyP-peroxidase from the alkaliphilic cellulomonad, Cellulomonas bogoriensis 82
Chapter VI Expression and characterization of a resurrected DyP-type peroxidase 100
Summary, Conclusion and Future Outlook 120
Nederlandse Samenvatting 126 Curriculum vitae 131 Acknowledgements 132 24 6 48 66 78 96 116 122 128 129
