University of Groningen
The molecular choreography of the Sec translocation system
Seinen, Anne-Bart
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Publication date: 2019
Link to publication in University of Groningen/UMCG research database
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Seinen, A-B. (2019). The molecular choreography of the Sec translocation system: From in vivo to in vitro. Rijksuniversiteit Groningen.
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The molecular choreography of
the Sec translocation system
From in vivo to in vitro
ISBN (printed version): 978-94-034-1394-5 ISBN (electronic version): 978-94-034-1395-2
The research described in this thesis was carried out at the Groningen Biomolecular Sciences and Biotechnology (GBB) institute and Zernike Institute of Advanced Materials of the University of Groningen, the Netherlands, and financially supported by the Foundation for Fundamental Research of Matter (FOM).
Cover design and layout by: Anne-Bart Seinen
On the cover: Stars representing single-molecules with a nebula of all the high-temporal SecA data clustered.
Printed by: Lovebird Design
Copyright © 2019 by Anne-Bart Seinen. All rights reserved. No part of this
publication may be reproduced, stored in a retrieval system, or transmitted in any form or by any means without the prior written permission of the author.
The molecular choreography of
the Sec translocation system
From in vivo to in vitro
Proefschrift
ter verkrijging van de graad van doctor aan de Rijksuniversiteit Groningen
op gezag van de
rector magnificus prof. dr. E. Sterken en volgens besluit van het College voor Promoties.
De openbare verdediging zal plaatsvinden op maandag 18 februari 2019 om 12.45 uur
door
Anne-Bart Seinen
geboren op 2 oktober 1986 te Hardenberg
Promotores
Prof. dr. A.J.M. Driessen Prof. dr. A.M. van Oijen Beoordelingscommissie
Prof. dr. B. Poolman Prof. dr. W.H. Roos Prof. dr. E.J.G. Peterman
Contents
Chapter 1 Single Molecule studies on the Protein Translocon 7
Chapter 2 Dynamics of membrane localization of the SecA 51 ATPase in Escherichia coli cells
Chapter 3 Dynamical organization of the Sec holotranslocon in 89 Escherichia coli cells
Chapter 4 Single-molecule observation of the ribosome-Sec translocon 129
interaction in planar bilayers
Chapter 5 Summary 157
Chapter 6 Samenvatting 161
