University of Groningen
Engineering amidases for peptide C-terminal modification Arif, Muhammad Irfan
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Publication date: 2018
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Arif, M. I. (2018). Engineering amidases for peptide C-terminal modification. University of Groningen.
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@ Muhammad Irfan Arif, Groningen,the Netherlands, 2018
ISBN (printed version):978-94-034-0948-1
ISBN (electronic version):978-94-034-0949-8
Graphicdesign and layout by the author.
The research done in this thesis was carried out at the Groningen Biomolecular Sciences and Biotechnology Institute (GBB) in the Biochemical Laboratory of the University of Groningen according to the requirements of the Graduate School of Science (Faculty of Mathematics and Natural Sciences, University of Groningen) and was supported by Netherlands Organization for Scientific Research (NWO). This project is part of the Integration of Biosynthesis and Organic Synthesis program (IBOS-2; project 053.63.014) funded by the Advanced Chemical Technologies for Sustainability (ACTS) and NWO.
Back Cover:River Chenabcrossingthrough Gujrat, Pakistan, photographed by the author.
Engineering amidases for peptide
C-terminal modification
PhD thesis
to obtain the degree of PhD at the University of Groningen
on the authority of the Rector Magnificus Prof. E. Sterken
and in accordance with
the decision by the College of Deans. This thesis will be defended in public on
Monday 8 October at 11:00 hours
by
Muhammad Irfan Arif
born on 19 March 1982 in Gujrat, Pakistan
Supervisor
Prof. D.B. Janssen
Assessment Committee
Prof. A.J.M. Driessen Prof. J. Kok
TABLE OF CONTENTS
Chapter 1 General Introduction: enzymatic synthesis of bioactive peptides 7
Chapter 2 Identification and characterization of a novel peptide amidase
from Glycin max (soybean)
89
Chapter 3 One-step C-terminal deprotection and activation of peptides with
peptide amidase from Stenotrophomonas maltophilia in neat organic solvent
113
Chapter 4 Peptide C-terminal modification using a computationally
stabilized peptide amidase
127
Chapter 5 Peptide esterification by SbPam: studies on organic solvent
stability and thermostability
153
Chapter 6 Summary and Outlook 175
Nederlandse samenvating 185
Acknowledgements 191
