University of Groningen
The molecular choreography of the Sec translocation system
Seinen, Anne-Bart
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Publication date: 2019
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Seinen, A-B. (2019). The molecular choreography of the Sec translocation system: From in vivo to in vitro. Rijksuniversiteit Groningen.
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Behorende bij het proefschrift
The molecular choreography of the Sec translocation system
From in vivo to in vitro
Anne-Bart Seinen
1. Single-molecule studies have come a long way and provide unprecedented details on highly dynamical processes. (Chapter 1) 2. SecA is homodimeric and predominantly bound to the cytoplasmic
membrane. (Chapter 2)
3. The Escherichia coli SecYEG channel is monomeric. (Chapter 3)
4. The holotranslocon exists and forms on demand. (Chapter 3)
5. With the right approach, isolating a subject and studying it in vitro can yield the same results as a native in vivo setting. (Chapter 4)
6. Image first, ask questions later.
7. Residing too long in your comfort zone gives a false sense of knowing stuff.
8. Meet’n is weet’n.
9. A camera can almost record anything; it is the analysis of the recorded that makes understanding difficult.
10. Scientific instruments nowadays are more and more automated – in a way this makes science easier and enables progress faster, but with
automation a lot of understanding is lost.
11. The pursuit of knowledge is hopeless and eternal. - Prof. H.J. Farnsworth 12. Scientists make excellent writers for television series and movies.