University of Groningen
Anatomy of the pneumococcal nucleoid
van Raaphorst, Renske
DOI:
10.33612/diss.127742005
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Publication date: 2020
Link to publication in University of Groningen/UMCG research database
Citation for published version (APA):
van Raaphorst, R. (2020). Anatomy of the pneumococcal nucleoid: Visualizing replication, chromosome segregation and chromosome condensation dynamics in Streptococcus pneumoniae. University of Groningen. https://doi.org/10.33612/diss.127742005
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Stellingen behorende bij het proefschrift
Anatomy of the pneumococcal nucleoid
Visualizing replication, chromosome segregation and chromosome condensation dynamics in Streptococcus pneumoniae
door Renske van Raaphorst
1. With the development of a whole artist’s palette of fluorescent proteins, it is important to test their functionality beyond E. coli (chapter 2, this thesis). 2. There is not such a thing as objective, unbiased cell segmentation based on
phase-contrast images only. Therefore, proper controls are vital (chapter 3 and 4, this thesis).
3. In the pneumococcus, DNA replication forks are not localized as proposed by the factory model (Dingman, 1971): the replication forks are highly mobile and observed as two separate units (chapter 3 and 4, this thesis).
4. Chromosome compaction as mediated by HU and SMC is an important driver for chromosome segregation in the pneumococcus (chapter 4 and 5, this thesis).
5. It is impossible to study the regulation of DNA replication, chromosome segregation or cell division in the pneumococcus as isolated events, since they happen simultaneously and influence each other (chapter 4, this thesis). 6. While early chromosome segregation is important for faultless septum placement, late chromosome segregation is probably vital for cell survival (chapter 4, 5 and 6, this thesis).
7. Beauty is a useful and often underestimated tool to get a point across (in scientific visualizations). Therefore, time spent on color scales is not necessarily wasted.
8. Whether it is in business, government or science, the fact that it is possible to gather data is no good reason to go ahead and do it.
9. With the variety of cell cycle regulation in microorganisms, one has to be careful with the generalization “in bacteria” in manuscript titles.
10. As long as people think “it is impressive you write your own code, especially since you are a woman” is a compliment, we have to keep working on gender balance in science.
