University of Groningen
Ether-lipid membrane engineering of Escherichia coli Caforio, Antonella
IMPORTANT NOTE: You are advised to consult the publisher's version (publisher's PDF) if you wish to cite from it. Please check the document version below.
Document Version
Publisher's PDF, also known as Version of record
Publication date: 2017
Link to publication in University of Groningen/UMCG research database
Citation for published version (APA):
Caforio, A. (2017). Ether-lipid membrane engineering of Escherichia coli. University of Groningen.
Copyright
Other than for strictly personal use, it is not permitted to download or to forward/distribute the text or part of it without the consent of the author(s) and/or copyright holder(s), unless the work is under an open content license (like Creative Commons).
Take-down policy
If you believe that this document breaches copyright please contact us providing details, and we will remove access to the work immediately and investigate your claim.
Downloaded from the University of Groningen/UMCG research database (Pure): http://www.rug.nl/research/portal. For technical reasons the number of authors shown on this cover page is limited to 10 maximum.
Ether-lipid membrane engineering
of Escherichia coli
Cover design and layout of the book: Antonella Caforio
Cover front and back: artistic interpretation of lipid rafts in a sea landscape. The lipid rafts are composed by two different lipid species representing archaeal and bacterial membrane lipds with the CarS structure embedded.
The research described in this thesis was carried out in the Department of Molecular Microbiology of the Groningen Biomolecular Sciences and Biotechnology Institute (GBB), University of Groningen, The Netherlands. It was financially supported by the biobased ecologically balanced sustainable industrial chemistry (BE-BASIC).
ISBN: 978-90-367-9379-7
ISBN: 978-90-367-9378-0 (electronic version)
Printed by Ipskamp printing, Enschede
Printing of this thesis was supported by generous contribution from the University of Groningen and the Groningen Biomolecular Sciences and Biotechnology Institute (GBB).
Copyright © 2016 by Antonella Caforio.
All rights reserved. No part of this thesis may be reproduced or transmitted in any form or by any means without the prior permission of the author.
Ether-lipid membrane
engineering of Escherichia coli
PhD thesis
to obtain the degree of PhD at the University of Groningen
on the authority of the Rector Magnificus Prof. E. Sterken
and in accordance with
the decision by the College of Deans. This thesis will be defended in public on
Friday 13 January 2017 at 14.30 hours by
Antonella Caforio
born on 14 April 1988 in Francavilla Fontana, Italy
Supervisor
Prof. A.J.M. Driessen
Assessment Committee
Prof. D.J. Slotboom Prof. O.P. Kuipers Prof. S. Schouten
A mio padre, mia madre e mia sorella per avermi sempre sostenuto,
incoraggiato e creduto in me dall’inizio alla fine.
Al mio amore, per avermi donato amore e felicitá.
Table of Contents
Chapter 1
Biosynthesis of archaeal membrane ether lipids…...9Chapter 2
Identification of CDP-archaeol synthase, a missinglink of ether lipid biosynthesis
in Archaea………...……...……..41
Chapter 3
Formation of the ether lipidsarchaeatidylglycerol and archaeatidyl-
ethanolamine in Escherichia coli………95
Chapter 4
Engineering of Escherichia coli with a hybridheterochiral membrane………129
Chapter 5
Structural insights into lipid biosynthesis inarchaeal membranes………..185
Chapter 6
Saturation of the ether lipids double bonds:Geranylgeranyl reductase………...217
