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University of Groningen

Exploring the biochemical and biocatalytic properties of bacterial DyP-type peroxidases

Colpa, Dana Irene

IMPORTANT NOTE: You are advised to consult the publisher's version (publisher's PDF) if you wish to cite from

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Document Version

Publisher's PDF, also known as Version of record

Publication date:

2018

Link to publication in University of Groningen/UMCG research database

Citation for published version (APA):

Colpa, D. I. (2018). Exploring the biochemical and biocatalytic properties of bacterial DyP-type peroxidases.

Rijksuniversiteit Groningen.

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Exploring the biochemical and

biocatalytic properties of bacterial

DyP-type peroxidases

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The research described in this thesis was carried out in the department of Molecular Enzymology of the Groningen Biotechnology and Biomolecular Sciences Institute (GBB) of the University of Groningen, according to the requirements of the Graduate School of Science, Faculty of Mathematics and Natural Sciences, University of Groningen, The Netherlands.

This work was financially supported by The Netherlands Organisation for Scientific Research (NWO) via the graduate program: Synthetic Biology for Advanced Metabolic engineering, project number 022.004.006.

Cover: The cover shows an artistic representation of the decolorization of dyes by a DyP-type peroxidase.

Cover design: Dana I. Colpa and Alexander Ziel

Printed by: Ipskamp Drukkers, Enschede (The Netherlands) ISBN: 978-94-034-1070-8 (printed version)

ISBN: 978-94-034-1069-2 (electronic version) Copyright © D.I. Colpa 2018, Groningen, The Netherlands.

All rights reserved. No part of this thesis may be reproduced, stored in a retrieval system, or transmitted in any form or by any means, without prior permission of the author.

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Exploring the biochemical and

biocatalytic properties of bacterial

DyP-type peroxidases

Proefschrift

ter verkrijging van de graad van doctor aan de

Rijksuniversiteit Groningen

op gezag van de

rector magnificus prof. dr. E. Sterken

en volgens besluit van het College voor Promoties.

De openbare verdediging zal plaatsvinden op

vrijdag 26 oktober 2018 om 11.00 uur

door

Dana Irene Colpa

geboren op 13 september 1988

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Promotor

Prof. dr. ir. M.W. Fraaije

Beoordelingscommissie

Prof. dr. D.B. Janssen

Prof. dr. G. Maglia

Prof. dr. L.O. Martins

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Chapter 1

Introduction and outline of the thesis

DyP-type peroxidases: a promising and versatile class

of enzymes

7

Chapter 2

Exploring the biocatalytic potential of a DyP-type

peroxidase by profiling the substrate acceptance of

Thermobifida fusca DyP peroxidase

25

Chapter 3

Exploring the catalytic properties of DyP-type

peroxidase TfuDyP by site-directed mutagenesis

43

Chapter 4

High overexpression of dye decolorizing peroxidase

TfuDyP leads to the incorporation of heme precursor

protoporphyrin IX

69

Chapter 5

Creating oxidase-peroxidase fusion enzymes as

tool-box for cascade reactions

85

Chapter 6

Conclusions and future perspectives

101

Chapter 7

Nederlandse samenvatting

111

Appendices

List of publications

123

Acknowledgements/Dankwoord

124

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