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University of Groningen

Let op! Cell wall under construction

Morales Angeles, Danae

IMPORTANT NOTE: You are advised to consult the publisher's version (publisher's PDF) if you wish to cite from

it. Please check the document version below.

Document Version

Publisher's PDF, also known as Version of record

Publication date:

2018

Link to publication in University of Groningen/UMCG research database

Citation for published version (APA):

Morales Angeles, D. (2018). Let op! Cell wall under construction: Untangling Bacillus subtilis cell wall

synthesis. University of Groningen.

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Let op!

Cell wall under construction:

Untangling Bacillus subtilis cell wall synthesis

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The work described in this thesis was carried out in the Department of Molecular Microbiology of the Groningen Biomolecular Science and Biotechnology Institute (GBB), University of Groningen, The Netherlands and was financially supported by the Dutch Science Organization (NWO)

ISBN (print): 9789403409337 ISBN (digital): 9789403409320

Printing of this thesis was supported by generous contribution from the University of Groningen and the Groningen Biomolecular Sciences and Biotechnology Institute.

Cover design: Oksana Cortes Tolalpa Layout: Lovebird desing

www.lovebird-desing.com

Copyright 2018 © by Danae Morales Angeles. All rights reserved. No part or this book may be reproduced, stored in a retrieval system, or transmitted in any form or by any means, wit-hout prior permission of the author.

Let op! Cell wall under construction

Untangling Bacillus subtilis cell wall synthesis

PhD thesis

to obtain the degree of PhD at the

University of Groningen

on the authority of the

Rector Magnificus Prof. E. Sterken

and in accordance with

the decision by the College of Deans.

This thesis will be defended in public on

Friday 12 October 2018 at 16.15 hours

by

Danae Morales Angeles

born on 23 February 1987

in Michoacán de Ocampo, Mexico

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TABLE OF CONTENTS

CHAPTER 1

The cell wall of Bacillus subtilis 7

INTRODUCTORY NOTE

Control experiments can trigger new stories

63

CHAPTER 2

In vivo cluster formation of nisin and Lipid II is correlated with

mem-brane depolarization

73

CHAPTER 3

Pentapeptide-rich peptidoglycan at the Bacillus subtilis cell-division site 89

CHAPTER 4

Unravelling the function of PBP2B PASTA domains

127

CHAPTER 5

Summary 159

Nederlandse samenvatting

167

Resumen 175

Acknowledment 183

List of publications

188

Supervisors

Prof. D.J. Scheffers

Prof. A.J.M. Driessen

Assessment Committee

Prof. D.J. Slotboom

Prof. J. Kok

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