University of Groningen
Characterisation of the M-locus and functional analysis of the male-determining gene in the
housefly
Wu, Yanli
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Publication date: 2018
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Wu, Y. (2018). Characterisation of the M-locus and functional analysis of the male-determining gene in the housefly. University of Groningen.
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Characterisation of the M-locus
and functional analysis of the
male-determining gene in the
housefly
The research described in this thesis was carried out in the Evolutionary Genetics group at the Centre for Ecology and Evolutionary Studies (CEES ) and from 2015 onwards at the Groningen Institute for Evolutionary Life Science (GELIFES) of the University of Groningen, the Netherlands and in the department of Developmental Biology at the Johann Friedrich Blumenbach Institute of Zoology and Anthropology, Georg August University, Göttingen, Germany, according to the requirements of the Graduate School of Science (Faculty of Science and Engineering, University of Groningen) and Georg-August University School of Science.
This work was funded by an Ubbo Emmius grant of the University of Groningen and by the Department of Developmental Biology at the Georg-August-University Göttingen. The printing of this thesis was supported by the University of Groningen and the Faculty of Science and Engineering. Cover design: Alexander Bentkamp
Printed by: Gildeprint, Enschede
ISBN: 978-94-034-0162-1 (printed version) 978-94-034-0161-4 (electronic version)
Characterisation of the M-locus
and functional analysis of the
male-determining gene in the
housefly
PhD thesis
to obtain the degree of PhD at the University of Groningen
on the authority of the Rector Magnificus Prof. E. Sterken
and in accordance with the decision by the College of Deans.
and
to obtain the degree of PhD at the University of Göttingen
on the authority of the President Prof. U. Beisiegel
and in accordance with
the decision by the Georg-August University School of Science. Double PhD degree
The thesis will be defended in public on Friday 5 January 2018 at 11.00 hours
by
Yanli Wu
born on 29 March 1988 in Yiwu, China
Supervisors Prof. L.W. Beukeboom Prof. E. A. Wimmer Co-supervisor Dr. L. van de Zande Assessment committee Prof. J. Kok Prof. G. Bucher Prof. G. Saccone Prof. Y. Huang
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Contents
Chapter 1
General introduction ... 7
Box 1.1
The common housefly, Musca domestica. ... 12
Box 1.2
The importance of studying Musca domestica for
pest control ... 16
Chapter 2
Characterisation of the complex nature of M-loci in
Musca domestica ... 23
Chapter 3
Comparative analysis of Mdmd genes located on
different chromosomes of Musca domestica ... 51
Chapter 4
Expression pattern and functional analysis of Mdmd
in Musca domestica ... 71
Box 4.1
Transgene construction for repeated expression of
Mdmd in Musca domestica ... 90
Chapter 5
General discussion ... 101
References ... 115
English summary ... 125
Nederlandse samenvatting ... 129
Deutsche Zusammenfassung ... 133
Acknowledgements ... 137
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