Targeted chemoprevention of head and neck cancer
de Boer, D.V.
2021
document version
Publisher's PDF, also known as Version of record
Link to publication in VU Research Portal
citation for published version (APA)
de Boer, D. V. (2021). Targeted chemoprevention of head and neck cancer.
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Chapter 4
Chemopreventive targeted treatment of head
and neck precancer by Wee1 inhibition
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Figure S1: Re-screening of 319 tumor-lethal siRNAs. a.2YHUYLHZRIWKHZRUNÁRZRIWKHUHVFUHHQ RI VL51$ 60$57SRROV VHOHFWHG IURP WZR JHQRPHZLGH VL51$ VFUHHQV RQ DQ H[WHQGHG FHOO OLQHSDQHORIWZHOYHWXPRUFHOOOLQHVRQHSUHFDQFHURXVFHOOOLQHDQGWKUHHSULPDU\ÀEUREODVWFXOWX UHV1H[WGDWDDQDO\VLVDQGVHOHFWLRQUHYHDOHGOHWKDOVL51$VRIZKLFKZHUHWXPRUVSHFLÀF )XUWKHUDQDO\VLVUHYHDOHGGUXJJDEOHJHQHVRIZKLFKZHUHHVVHQWLDOLQWKHSUHFDQFHURXVFHOOVDV ZHOO7DUJHWHGWKHUDS\ZDVH[SORUHGE\FROODWHUDOOHWKDOLW\DVZHOODVYDOLGDWLRQRIRQHVSHFLÀFWDUJHW JHQH:((b.*UDSKLFDOSUHVHQWDWLRQRIWKHUHVFUHHQLQJRIWKHH[WHQGHGFHOOOLQHSDQHOZLWKWKH VL51$60$57SRROV&HOOVZHUHHLWKHUIRUZDUGO\WUDQVIHFWHGKDIWHUVHHGLQJRUUHYHUVHO\E\ WUDQVIHFWLQJFHOOVGXULQJVHHGLQJ$IWHUKRIVL51$WUDQVIHFWLRQFHOOYLDELOLW\ZDVPHDVXUHGXVLQJ &HOO7LWHU%OXHc.0HDQQXPEHURIOHWKDOVL51$60$57SRROVSHUJURXSRIFHOOOLQHV7KHSULPDU\RUDO ÀEUREODVWVVKRZHGRYHUWKUHHWLPHVOHVVOHWKDOKLWVFRPSDUHGWRWKH+16&&FHOOOLQHJURXSV
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Gene CCH- FA HN-SCC-2 VU- SCC- 1131 VU- SCC- 1365 VU- SCC- 1604 VU-SCC -120 (refer ence ) VU- SCC- OE UM- SCC- 11B UM- SCC- 22B UM- SCC- 104 UM- SCC- 47 UT -SCC- 45 VU- SCC- 147 Fibro- blasts #50 Fibro- blasts #54 Fibro- blasts VU-1678CCDC99 CCT4 CCT7 CCT8 CD207 CDC40 CDC5L CDCA8 CDK1 CDK1 1A CELF5 CENPE CEP72 CHMP2A CKAP5 COL10A1 COL20A1 COP A COPB1 COPB2 COPG1 na COPZ1 COPZ2 CRNKL1 CSDC2 CSNK2A2 CST A na CWC15 T
able S1: Median of triplicate viability scores of siRNAs per cell line
#. Gene CCH- FA HN-SCC-2 VU- SCC- 1131 VU- SCC- 1365 VU- SCC- 1604 VU-SCC -120 (refer ence ) VU- SCC- OE UM- SCC- 11B UM- SCC- 22B UM- SCC- 104 UM- SCC- 47 UT -SCC- 45 VU- SCC- 147 Fibro- blasts #50 Fibro- blasts #54 Fibro- blasts VU-1678
ADAMTS17 * AGFG2 AHSP ƾ ALDH18A1 ANAPC1 ANAPC4 AP5S1 na APCDD1L Ȼ AQR ARCN1 ARFGEF1 ARHGAP20 ARL14 ARSG ASB16 ASNS na A T P6AP1 BIRC5 C14orf129 C19orf59 C22orf29 C8orf31 CACTIN CALCOCO2 CCBL2 CCDC153 T able S1 (continued)