University of Groningen
Targeted diazotransfer to proteins
Lohse, Jonas
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Publication date: 2018
Link to publication in University of Groningen/UMCG research database
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Lohse, J. (2018). Targeted diazotransfer to proteins. University of Groningen.
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Cover design: Andrej Horoschun, https://horoschun.artstation.com/ ISBN (print) 978-94-034-0733-3
ISBN (digital) 978-94-034-0732-6
Printed by Gildeprint Drukkerijen, Enschede, The Netherlands The work described in this thesis was carried out in the Chemical Biology workgroup at the Stratingh Institute for Chemistry, University of Groningen, The Netherlands
The work was financially supported by The Netherlands Organisation for Scientific Research (NWO)
Targeted Diazotransfer to
Proteins
PhD Thesis
To obtain the degree of PhD at the
University of Groningen
on the authority of the
Rector Magnificus Prof. E. Sterken
and in accordance with
the decision by the College of Deans.
The thesis will be defended in public on
Friday, 8 June 2018 at 14:30 hours
By
Jonas Lohse
born on 17 October 1986
in Hamburg City, GermanySupervisors
Prof. Dr. Ir. A.J. Minnaard
Prof. Dr. M.D. Witte
Assessment Committee
Prof. Dr. J.G. Roelfes
Prof. Dr. Ir. M.W. Fraaije
Prof. Dr. Ir. J.C.M. van Hest
Table of contents
Chapter 1 11
A general Introduction to chemical biology, bioorthogonal chemistry and the introduction of xenobiotic groups to biomolecules.
Chapter 2 57
A proof-of-concept study for targeted diazotransfer probes: modifying biotin binding proteins.
Chapter 3 93
Targeted diazotransfer probes in combination with a clickable and cleavable linker (clinker) instrumental to target deconvolution.
Chapter 4 109
Targeted diazotransfer probes for the modification of carbonic anhydrase II: on the origin of efficiency, selectivity and site-specificity.
Chapter 5 155
A modular approach for the construction of protein targeting probes: a methodology to synthesise probes based on imine chemistry.
Appendix
Summary 182
Nederlandse Samenvatting 186
If we are to preserve culture, we must continue to create it.
