University of Groningen
Production routes toward podophyllotoxin
Seegers, Christina
DOI:
10.33612/diss.168957811
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Publication date: 2021
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Citation for published version (APA):
Seegers, C. (2021). Production routes toward podophyllotoxin. University of Groningen. https://doi.org/10.33612/diss.168957811
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PRODUCTION ROUTES
toward
Podophyllotoxin
The research described in this thesis was carried out in the Department of Chemical and Pharmaceutical Biology (Groningen Research Institute of Pharmacy, University of Groningen, the Netherlands) according to the requirements of the Graduate School of Science (Faculty of Science and Engineering, University of Groningen, the Netherlands).
This work was supported by European and regional funding. The PhytoSana project in the INTERREG IV A Deutschland-Nederland program: 34- INTERREG IV A I-1-01=193; and the Disposable Bioreactoren project in the Innovatief Actieprogramma Groningen: IAG3-D02.
Printing of this thesis was financially supported by the University Library and the Graduate School of Science, Faculty of Science and Engineering, University of Groningen, the Netherlands.
Cover design & illustrations: Ilse Schrauwers - isontwerp.nl Layout: Christel Seegers
Printed by: Gildeprint - www.gildeprint.nl ISBN: 978-94-92303-41-7
Copyright © 2021 by Christel Seegers. All rights reserved. No parts of this thesis may be reproduced or transmitted in any form or by any means without the prior permission in writing of the author.
Production routes toward
podophyllotoxin
Proefschrift
ter verkrijging van de graad van doctor aan de Rijksuniversiteit Groningen
op gezag van de
rector magnificus prof. dr. C. Wijmenga en volgens besluit van het College voor Promoties.
De openbare verdediging zal plaatsvinden op vrijdag 28 mei 2021 om 12.45 uur
door
Christina Lamberta Catharina Seegers
geboren op 11 juni 1989 te Grubbenvorst
Promotor Prof. dr. W.J. Quax Beoordelingscommissie Prof. dr. K. Taxis Prof. dr. J.T.M. Elzenga Prof. dr. S. Sukrasno
5
Table of contents
Chapter 1 Introduction and scope of this thesis 7 Chapter 2 Toward metabolic engineering of podophyllotoxin
production
15
Chapter 3 Transcriptome analysis of Podophyllum hexandrum and functional expression of cytochrome P450 monooxygenases and NADPH-cytochrome P450 reductases in Escherichia coli
39
Chapter 4 Cytotoxic deoxypodophyllotoxin can be extracted in high purity from Anthriscus sylvestris roots by supercritical carbon dioxide
71
Chapter 5 Disposable bioreactor with integrated oxygen sensors for production of secondary metabolites in plant cell cultures
95
Chapter 6 Methyl jasmonate treatment increases podophyllotoxin production in Podophyllum hexandrum roots under glasshouse conditions
111
Chapter 7 Summary and future perspectives 133 Appendix Nederlandse samenvatting
List of abbreviations List of publications Curriculum Vitae Dankwoord
Chapter X
