University of Groningen Single-molecule fret study on structural dynamics of membrane proteins Aminian Jazi, Atieh

University of Groningen

Single-molecule fret study on structural dynamics of membrane proteins Aminian Jazi, Atieh

DOI:

10.33612/diss.135802718

IMPORTANT NOTE: You are advised to consult the publisher's version (publisher's PDF) if you wish to cite from it. Please check the document version below.

Document Version

Publisher's PDF, also known as Version of record

Publication date: 2020

Link to publication in University of Groningen/UMCG research database

Citation for published version (APA):

Aminian Jazi, A. (2020). Single-molecule fret study on structural dynamics of membrane proteins. University of Groningen. https://doi.org/10.33612/diss.135802718

Copyright

Other than for strictly personal use, it is not permitted to download or to forward/distribute the text or part of it without the consent of the author(s) and/or copyright holder(s), unless the work is under an open content license (like Creative Commons).

Take-down policy

If you believe that this document breaches copyright please contact us providing details, and we will remove access to the work immediately and investigate your claim.

Downloaded from the University of Groningen/UMCG research database (Pure): http://www.rug.nl/research/portal. For technical reasons the number of authors shown on this cover page is limited to 10 maximum.

Single-Molecule FRET Study on Structural Dynamics

of Membrane Proteins

Atieh Aminian 2020

2

Atieh Aminian PhD thesis

University of Groningen, The Netherlands

Zernike Institute for Advanced Materials PhD thesis series 2020-14

ISBN:978-90-830-7040-7

ISSN:1570-1530

The research described on this thesis was carried out in University of Groningen in the Zernike Institute of Advanced Materials of University of Groningen, the Netherlands . This work was financed by the Zernike Institute for Advanced Materials, the Centre for Synthetic Biology and ERC Starting Grant (ERC-STG 638536 – SM-IMPORT to T.C.). Atieh Aminian was supported by Ubbo Emmius funding (University of Groningen) and by the DFG in the frame of SFB699. E.P. was supported by a DFG fellowship (PL696/2-1).

Cover Design by: Atieh Aminian

Copyright © Atieh Aminian 2020, Groningen, The Netherlands.

All right reserved, no part of this publication may be produced, stored in retrieval system, or transmitted in any form or by any means without prior written permission of the author.

3

Single-Molecule FRET Study on

Structural Dynamics of Membrane

Proteins

Proefschrift

ter verkrijging van de graad van doctor aan de Rijksuniversiteit Groningen

op gezag van de

rector magnificus prof. dr. C. Wijmenga en volgens besluit van het College voor Promoties.

De openbare verdediging zal plaatsvinden op dinsdag 2 juni 2020 om 9.00 uur

door

Atieh Aminian Jazi

geboren op 04 September 1987 Tehran, Iran

4

Promotores

Copromotor

Beoordelingscommissie

5

Contents

Chapter 1: 6

Introduction & Background

Chapter 2: 25

Fluorescence labelling of membrane transporters and isolated protein domains

Chapter 3: 48

Caging and Photoactivation in Single-molecule Förster-resonance energy transfer Experiments

Chapter 4: 81

smFRET study on the potassium-induced conformational changes of the C-terminal sensory domain in the betaine transporter BetP

Chapter 5: 123

Summary

Samenvatting 129