University of Groningen
Single-molecule studies of the conformational dynamics of ABC proteins
de Boer, Marijn
DOI:
10.33612/diss.125779120
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Publication date: 2020
Link to publication in University of Groningen/UMCG research database
Citation for published version (APA):
de Boer, M. (2020). Single-molecule studies of the conformational dynamics of ABC proteins. University of Groningen. https://doi.org/10.33612/diss.125779120
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Single-molecule studies of the
conformational dynamics of ABC proteins
Printing: GVO drukkers & vormgevers B.V. Zernike Institute PhD thesis series: 2020-10
ISSN: 1570-1530
ISBN: 978-94-034-2641-9 (printed version) ISBN: 978-94-034-2640-2 (electronic version)
The research described in this thesis was carried out at the Zernike Institute for Advanced Materials (ZIAM) of the University of Groningen, the Netherlands, and financially supported by the European Research Council (ERC).
Copyright © 2020 Marijn de Boer. All rights reserved. No part of this publication may be reproduced, stored in a retrieval system, or transmitted in any form or by any means without the prior written permission of the author.
Single-molecule studies of the
conformational dynamics of ABC
proteins
Proefschrift
ter verkrijging van de graad van doctor aan de Rijksuniversiteit Groningen
op gezag van de
rector magnificus prof. dr. C. Wijmenga en volgens besluit van het College voor Promoties.
De openbare verdediging zal plaatsvinden op dinsdag 2 juni 2020 om 11.00 uur
door
Marijn Johan de Boer
geboren op 30 juli 1987 te Zaanstad
Promotores
Prof. dr. T. Cordes
Prof. dr. ir. E. van der Giessen
Copromotor
Dr. G. GkouridisBeoordelingscommissie
Prof. dr. W.H. Roos Prof. dr. P. Tinnefeld Prof. dr. E. Bordignon5
Contents
1 General introduction 7
2 Conformational and dynamic plasticity in substrate-binding proteins underlies selective transport in ABC importers
33
3 Mathematical treatment of transport inhibition in ABC importers 77 4 Conformational states of the substrate-binding proteins SiaP and CynR
probed by single-molecule FRET
107
5 Single-molecule observation of ligand binding and conformational changes in FeuA
121
6 Protein linkers provide limits on the domain interactions in the ABC importer GlnPQ and determine the rate of transport
149
7 ABCE1 controls ribosome recycling by an asymmetric dynamic conformational equilibrium
169
8 The relation between intrinsic protein conformational changes and ligand binding
201
9 Summary and outlook 223
10 Nederlandse samenvatting 233
