University of Groningen
Pathogenic, versatile and tunable activity of sortase, a transpeptidation machine
Wójcik, Magdalena
DOI:
10.33612/diss.119637108
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Publication date: 2020
Link to publication in University of Groningen/UMCG research database
Citation for published version (APA):
Wójcik, M. (2020). Pathogenic, versatile and tunable activity of sortase, a transpeptidation machine. https://doi.org/10.33612/diss.119637108
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Pathogenic, versatile and
tunable activity of sortase,
a transpeptidation machine
The research described in this thesis was carried out in the Department of Chemical and Pharmaceutical Biology, part of the Groningen Research Institute of Pharmacy (GRIP), at the University of Groningen, in The Netherlands. Research was funded by the Rosalind Franklin Fellowship.
ISBN: 978-94-034-2489-7 (Printed book) ISBN: 978-94-034-2490-3 (Ebook) Cover design: Magdalena Wójcik
Layout and design by Vera van Ommeren, persoonlijkproefschrift.nl. Printing: Ridderprint | www.ridderprint.nl
Copyright © 2020 Magdalena Wójcik. All rights are reserved. No part of this thesis may be reproduced or transmitted in any form or by any means without the prior permission in writing of the author.
Pathogenic, versatile and tunable
activity of sortase,
a transpeptidation machine
PhD thesis
to obtain the degree of PhD at the University of Groningen
on the authority of the Rector Magnificus Prof. C. Wijmenga
and in accordance with the decision by the College of Deans. This thesis will be defended in public on
Friday 17 April 2020 at 16.15 hours by
Magdalena Wójcik born on 14 December 1988
Supervisor
Prof. W.J. Quax
Co-supervisor
Dr. Y.L. Boersma
Assessment Committee
Prof. J.M. van Dijl Prof. M.R. Groves Prof. U. Schwaneberg
TABLE OF CONTENTS
CHAPTER 1 Introduction and scope of the thesis
CHAPTER 2 Identification of potential antivirulence agents by
substitution-oriented screening for inhibitors of
Streptococcus pyogenes Sortase A
CHAPTER 3 Improvement of transpeptidation activity of
Streptococcus pyogenes sortase A by modelling and
iterative saturation mutagenesis
CHAPTER 4 Engineering the specificity of Streptococcus pyogenes
sortase A by loop grafting
CHAPTER 5 Sortase mutants with improved protein
thermostability and enzymatic activity obtained by consensus design
CHAPTER 6 High-Throughput Screening in Protein Engineering:
Recent Advances and Future Perspectives
CHAPTER 7 Adaptation of Cellular High-throughput
Encapsulation Solubilization and Screening (CHESS) for Staphylococcus aureus sortase A
CHAPTER 8 Summary, general discussion and future outlook
DUTCH SUMMARY ACKNOWLEDGEMENTS 7 13 37 61 77 101 137 157 164 170
