University of Groningen
Exploiting the Biomolecular Corona of Nanocarriers for Targeted drug Delivery to Endothelial Cells
Aliyandi, Aldy
DOI:
10.33612/diss.133166778
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Publication date: 2020
Link to publication in University of Groningen/UMCG research database
Citation for published version (APA):
Aliyandi, A. (2020). Exploiting the Biomolecular Corona of Nanocarriers for Targeted drug Delivery to Endothelial Cells. University of Groningen. https://doi.org/10.33612/diss.133166778
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Exploiting the Biomolecular Corona of
Nanocarriers for Targeted Drug
Delivery to Endothelial Cells
Aldy Aliyandi
2020
Paranymphs:
Simon Spahrkäs
Dulce Pamela Gonzalez Davila
Cover design: Aldy Aliyandi Layout design: Aldy Aliyandi Printed by: Proefschrift-AIO
ISBN: 978-94-93184-58-9
Copyright © 2020, Aldy Aliyandi
The research presented in this thesis was performed in the department of Nanomedicine and Drug Targeting at the Groningen Research Institute of Pharmacy (GRIP), University of Groningen, the Netherlands, and financially supported by GRIP and partly by the European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation programme under grant agreement No 637614 (NanoPaths). Printing of this thesis was
financially supported by the University Library and the Graduate School of Science and Engineering, Faculty of Science and Engineering, University of Groningen, the Netherlands
All right reserved. No part of this thesis may be reproduced in any form or by any means without prior permission of the author and the publisher holding the copyright of the published articles.
Exploiting the Biomolecular Corona
of Nanocarriers for Targeted Drug
Delivery to Endothelial Cells
PhD Thesis
to obtain the degree of PhD at the University of Groningen
on the authority of the
Rector Magnificus Prof. C. Wijmenga and in accordance with
the decision by the College of Deans. This thesis will be defended in public on
Monday 5 October 2020 at 11.00 hours
by
Aldy Aliyandi
born on 2 December 1991 in Bandung, Indonesia
Supervisors
Prof. A. Salvati Prof. I. S. ZuhornAssessment Committee
Prof. W. Parak Prof. G. Molema Prof. H. W. FrijlinkTable of Contents
Chapter 1. General Introduction and Aim of the Thesis ...9
1.1 Nanomedicine ... 10
1.2 Endothelial cell barriers in nanomedicine ... 18
1.3 Aim of the thesis ... 20
References ... 24
Chapter 2. Disentangling Biomolecular Corona Interactions with Cell Receptors and Implications for Targeting of Nanomedicines ... 33
2.1 Introduction ... 34
2.2 The role of receptors in nanoparticle uptake ... 36
2.3 The role of the biomolecular corona in nanoparticle uptake ... 37
2.4 Methods to elucidate nanoparticle biomolecular corona-receptor interactions ... 40
2.5 Conclusions and future perspectives ... 49
References ... 51
Chapter 3. Effect of the Development of a Cell Barrier on Nanoparticle Uptake in Endothelial Cells ... 57
3.1 Introduction ... 58
3.2 Materials and methods ... 60
3.3 Results and discussion ... 67
3.4 Conclusions ... 78
Acknowledgements ... 79
References ... 81
Supplementary Information ... 88
Chapter 4. Effect of Endothelial Cell Heterogeneity on Nanoparticle Uptake ... 113
4.1 Introduction ... 114
4.2 Materials and methods ... 116
4.3 Results ... 121 4.4 Discussion ... 128 4.5 Conclusions ... 132 Acknowledgements ... 132 References ... 134 Supplementary Information ... 139
Chapter 5. Correlating Corona Composition and Cellular Uptake to Discover Proteins Directing Nanoparticles into Cells... 145
5.1 Introduction ... 146
5.2 Materials and methods ... 148
5.3 Results and discussion ... 154
5.4 Conclusions ... 164
Acknowledgements ... 165
References ... 167
Supplementary Information ... 171
Chapter 6. Exploiting the Protein Corona to Identify Receptors for Targeting of Nanocarriers ... 179
6.1 Introduction ... 180
6.2 Materials and methods ... 182
6.3 Results and discussion ... 188
6.4 Conclusions ... 202
Acknowledgements ... 203
References ... 205
Supplementary Information ... 209
Chapter 7. General Discussion and Future Perspectives ... 227
References ... 238
Nederlandse Samenvatting ... 240
