University of Groningen
Epidemiology, genetic diversity and clinical manifestations of arboviral diseases in Venezuela
Lizarazo, Erley F.
DOI:
10.33612/diss.108089934
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Publication date: 2019
Link to publication in University of Groningen/UMCG research database
Citation for published version (APA):
Lizarazo, E. F. (2019). Epidemiology, genetic diversity and clinical manifestations of arboviral diseases in Venezuela. University of Groningen. https://doi.org/10.33612/diss.108089934
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Epidemiology, genetic diversity and
clinical manifestations of arboviral
diseases in Venezuela
This PhD project was performed at the Institute of Biomedical Investigation "Dr. Francisco J. Triana Alonso" within the University of Carabobo (Biomed-UC), Venezuela and at the Department of Medical Microbiology and Infection Prevention of the University Medical Center Groningen (UMCG), University of Groningen, The Netherlands.
This work was supported by the Fondo Nacional de Ciencia y Tecnología e Innovación (FONACIT), grant 201100129, 201300201; by the Coordinación de Aplicación de Fondos e Incentivos para el Desarrollo de Planes de Ciencia, Tecnología e Innovación, Organic Law of Science, Technology and Innovation (LOCTI), certification No DGCAFIDCTI/204-214-10, of the Venezuelan Ministry of Science, Technology and Innovation, Venezuela. E. F. Lizarazo Forero received an Abel Tasman Talent Program grant of the Groningen University Institute for Drug Exploration (GUIDE) and financial support from the Department of Medical Microbiology and Infection Prevention, UMCG, University of Groningen, Groningen, The Netherlands.
The printing of this thesis was financially supported by:
ISBN:
978-94-034-2168-1 (Printed book) 978-94-034-2167-4 (Digital)
Cover design:
Maria F. Vincenti-González / Erley Lizarazo
Cover Illustrations
Wright-Fisher Haploid Model / Dengue structure model from Protein Data Bank
Design/Layout:
Ioana Margineanu / Erley Lizarazo
Print:
IPSKAMP printing
© Erley Ferlipe Lizarazo Forero
All rights reserved. No part of this publication may be reproduced or transmitted in any form or by any means without written permission of the author and, when appropriate, the publisher holding the copyrights of the published articles.
Epidemiology, genetic diversity and
clinical manifestations of arboviral
diseases in Venezuela
PhD thesis
to obtain the degree of PhD at the
University of Groningen
on the authority of the
Rector Magnificus Prof. C. Wijmenga
and in accordance with
the decision by the College of Deans.
This thesis will be defended in public on
Wednesday 11 December 2019 at 14.30 hours
by
Erley Ferlipe Lizarazo Forero
born on 5 December 1987
in La Fría, Venezuela
Supervisors
Prof. J.W.A Rossen
Prof. A.W. Friedrich
Co-supervisor
Dr. A. Tami
Dr. M.E. Grillet
Assessment Committee
Prof. P. Mayaud
Prof. M. de Jong
Prof. J.M. Smit
Paranymphs
Paola Lisotto
Erwin Raangs
General Introduction and Scope of the Thesis
6
General Introduction and Scope of the Thesis
7
General Introduction and Scope of the Thesis
8
General Introduction and Scope of the Thesis
9
General Introduction and Scope of the Thesis
10
Chapter 1
11
General Introduction and Scope of the Thesis
12
1
Table of contents
Chapter 1 General Introduction and Scope of the Thesis 9
Chapter 2 Spatial dynamics of chikungunya virus in Venezuela: The first
six months of the epidemic 29
Chapter 3 Concomitant chikungunya and dengue epidemic in Carabobo
State, Venezuela 2014: a study on epidemiological development, clinical manifestations and risk factors
63
Chapter 4 Applied shotgun metagenomics approach for the genetic
characterization of dengue viruses 89
Chapter 5 Complete coding sequences of five dengue virus type 2
clinical isolates from Venezuela obtained through shotgun metagenomics
117
Chapter 6 DEN-IM: Dengue virus identification from metagenomic and
targeted sequencing data 123
Chapter 7 Evolutionary history and population dynamics of dengue
viruses in Venezuela 157
Chapter 8 Summarizing Discussion 193
Appendix Thesis summary
Nederlandse Samenvatting
Informed Consent and Survey Instruments Acknowledgments Curriculum Vitae 210 214 218 242 246
