Cover Page

The handle

http://hdl.handle.net/1887/133432

holds various files of this Leiden University

dissertation.

Author: Akram, M.N.

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Figure 7.1. 1_{H-NMR spectra (500 MHz) of phosphate buffer (pH 6.0) - MeOD (1:1)}

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two years old bulbs and C) three years old bulbs without the smaller out growing daughter bulbs.

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Table 7.1. Chemical shifts ƂDQGFRXSOLQJFRQVWDQWs (Hz) of identified metabolites in NMR of phosphate buffer (pH 6.0) - MeOD (1:1) extracts of Narcissus. Numbers correlate to signals in Figure 7.1

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Figure 7.2. A) Score plot of PCA (PC1 vs PC2) of the 1_{H-NMR-spectra of Narcissus}

pseudonarcissus Cultivar ‘Carlton’ bulbs (Figure 7.1) where numbers represent the age

of the bulb.

B) Loadings scatter plot of PCA (PC1 vs PC2) of Narcissus bulbs. Compound abbreviations as in TTable 7.1.

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D) Loadings scatter plot of PLS-DA (z) Loadings (Ŷ) average of a classes. PLS-DA models were validated by using permutation test (R2 _{= 0.82, Q}2 _{= 0.70 with 120}

permutations and 8 components)

Figure 7.3. Relative quantitation of some compounds in different Narcissus

pseudonarcissus Cultivar ‘Carlton’ bulbs calculated from the 500 MHz 1_{H-NMR spectra.}

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the significant comparison such as (aa) denotes significant difference when compared with one-year and two-year old bulbs and (bb) denotes significant difference when compared with three-year or three-year mother bulbs.

Table 7.2. Molecular ion and fragment ions of the alkaloids of Narcissus pseudonarcissus Cultivar ‘Carlton’ with retention time from GC-MS

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Figure 7.4. A standard curve for galanthamine hydrobromide on GC-FID obtained with a dilution series where each measuring point is based on three replicates.

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Figure 7.6. Quantitative GC-FID analysis alkaloid contents of Narcissus pseudonarcissus Cultivar ‘Carlton’ bulbs of different age. GAL, galanthamine (P = .01582); HAE, haemanthamine (P = .02687); HOMO, homolycorine (P = .03454); LYC, lycorenine (P = .01747); TAZ, tazettine (P = .10574) (5 biological replicates, significance at P < .05, error bars represent standard error (SEM). Different letters show the significant comparison such as (aa) denotes significant difference when compared with one-year old bulbs and (b) denotes significant difference when compared with two-year and three-year old bulbs.

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