Cover Page
The following handle holds various files of this Leiden University dissertation:
http://hdl.handle.net/1887/60912
Author: Bruin, R.G. de
Title: The RNA-binding protein Quaking in vascular health and disease
Issue Date: 2018-04-05
The RNA-binding protein Quaking in vascular health and disease
R.G. de Bruin
The RNA-binding protein Quaking in vascular health and disease ISBN: 978-94-028-0944-2
© R.G. de Bruin 2018
© Cover photograph: T.D. Matngelsen, USA, printed with permission
All rights are reserved. No part of this publication may be reproduced, stored in a retrieval system, or transmitted in any form or by any means, without permission of the copyright owners.
The research presented in this thesis was performed at the Einthoven Laboratory of Experimental Vascular Medicine, Division of Nephrology, Department of Internal Medicine, Leiden University Medical Center, The Netherlands.
And the Lady Davis Institute for Medical Research, McGill University, Montréal, Canada.
The RNA-binding protein Quaking in vascular health and disease
Proefschrift
ter verkrijging van
de graad van Doctor aan de Universiteit Leiden
op gezag van de Rector Magnificus prof. mr. C.J.J.M. Stolker volgens besluit van het College voor Promoties
te verdedigen op donderdag 5 april 2018 klokke 16.15 uur
door
Ruben Gosewinus de Bruin
geboren te 's-Gravenhage in 1987
Promotiecommissie
Promotor Prof. dr. A.J. van Zonneveld
Copromotor Dr. E.P. van der Veer
Overige leden Prof. Dr. A.J. Rabelink Prof. Dr. P.H. Reitsma Prof. Dr. D.E. Atsma
Prof. Dr. H. van Esch, Leuven University, Belgium Prof. Dr. S. Richard, McGill University, Canada dr. J.M. van Gils
Financial support:
Medisch Specialistisch Bedrijf van het Groene Hart Ziekenhuis
Financial support by the Dutch Heart Foundation for the publication of this thesis is gratefully acknowledged The cover of this thesis was generously provided by Thomas D. Mangelsen, http://mangelsen.com
Opgedragen aan Dick Gosewinus de Bruin
Table of contents
Chapter 1 Preface: English and Dutch summary 9
Chapter 2 Scope of this thesis 13
Chapter 3 Introduction to cardiovascular disease and RNA biology 17
Chapter 4 The RNA-binding protein Quaking 29
Chapter 5 Emerging roles for RNA-binding proteins as effectors and 51
regulators of cardiovascular disease European Heart Journal 2016
Chapter 6 Quaking, an RNA-Binding Protein, Is a Critical Regulator of 77
Vascular Smooth Muscle Cell Phenotype Circulation Research 2013
Chapter 7 Quaking promotes monocyte differentiation into pro- 105
atherogenic macrophages by controlling pre-mRNA splicing and gene expression
Nature Communications 2016
Chapter 8 The RNA-binding protein quaking maintains endothelial 149
barrier function and affects VE-cadherin and β-catenin protein expression.
Scientific Reports 2016
Chapter 9 Targeting Quaking ameliorates macrophage-induced 171
renal interstitial fibrosis and induces alternative splicing of pre-mRNA transcripts in differentiated macrophages Manuscript in preparation
Chapter 10 Discussion & future directions 193
Chapter 11 Appendices 209
Curriculum Vitae Bibliography Dankwoord
