University of Groningen
Fructose-1,6-bisphosphate and its role on the flux-dependent regulation of metabolism
Bley Folly, Brenda
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Publication date: 2018
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Bley Folly, B. (2018). Fructose-1,6-bisphosphate and its role on the flux-dependent regulation of metabolism. University of Groningen.
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Fructose-1,6-bisphosphate and its role on the
flux-dependent regulation of metabolism
Brenda Bley Folly
2017
Fructose-1,6-bisphosphate and its
role on the flux-dependent
regulation of metabolism
Brenda Bley Folly
2017
The work published on this thesis was carried out at the Molecular Systems Biology research group of the Groningen Biomolecular Sciences and Biotechnology insti tute (GBB) at the University of Groningen, The Netherlands.
This work was fi nancially supported by the Science without Borders program, from the Brazilian Nati onal Council for Scienti fi c and Technological Development (CNPq), process 245630/2012-0.
ISBN: 978-94-034-0338-0 (printed version) ISBN: 978-94-034-0339-7 (electronic version) Printed by: Proefschrift maken, The Netherlands Cover: Picture by Freepik. Design by Ron Luchies.
Copyright © 2017 by Brenda Bley Folly. All rights reserved. No part of this thesis may be reproduced, stored in a retrieval system or transmitt ed in any form or by any means without the prior writt en permission of the author.
The work published on this thesis was carried out at the Molecular Systems Biology research group of the Groningen Biomolecular Sciences and Biotechnology institute (GBB) at the University of Groningen, The Netherlands.
This work was financially support by the ScienceÊwithoutÊBorders program, from the Brazilian National Council for Scientific and Technological Development (CNPq), process 245630/2012-0.
ISBN: 978-94-034-0338-0 (printed version) ISBN: 978-94-034-0339-7 (electronic version)
Printed by: Proefschriftmaken, The Netherlands
Copyright © 2017 by Brenda Bley Folly. All rights reserved. No part of this thesis may be reproduced, stored in a retrieval system or transmitted in any form or by any means without the prior written permission of the author.
Fructose-1,6-bisphosphate and its
role on the flux-dependent
regulation of metabolism
PhD thesis
to obtain the degree of PhD at the University of Groningen
on the authority of the Rector Magnificus Prof. E. Sterken
and in accordance with the decision by the College of Deans. This thesis will be defended in public on
Friday 19 January 2018 at 09.00 hours
by
Brenda Bley Folly
born on 14 November 1983Fructose-1,6-bisphosphate and its role on the fl ux-dependent regulation of metabolism
PhD thesis
to obtain the degree of PhD at the University of Groningen
on the authority of the Rector Magnifi cus Prof. E. Sterken
and in accordance with the decision by the College of Deans. This thesis will be defended in public on
Friday 19 January 2018 at 09.00 hours
by
Brenda Bley Folly born on 14 November 1983
in Curitiba, Brazil
The work published on this thesis was carried out at the Molecular Systems Biology research group of the Groningen Biomolecular Sciences and Biotechnology institute (GBB) at the University of Groningen, The Netherlands.
This work was financially support by the ScienceÊwithoutÊBorders program, from the Brazilian National Council for Scientific and Technological Development (CNPq), process 245630/2012-0.
ISBN: 978-94-034-0338-0 (printed version) ISBN: 978-94-034-0339-7 (electronic version)
Printed by: Proefschriftmaken, The Netherlands
Copyright © 2017 by Brenda Bley Folly. All rights reserved. No part of this thesis may be reproduced, stored in a retrieval system or transmitted in any form or by any means without the prior written permission of the author.
Fructose-1,6-bisphosphate and its
role on the flux-dependent
regulation of metabolism
PhD thesis
to obtain the degree of PhD at the University of Groningen
on the authority of the Rector Magnificus Prof. E. Sterken
and in accordance with the decision by the College of Deans. This thesis will be defended in public on
Friday 19 January 2018 at 09.00 hours
by
Brenda Bley Folly
born on 14 November 1983Supervisor
Prof. M. Heinemann
Assessment Committee
Prof. D.J. Slotboom
Prof. M.W. Fraaije
Prof. P. Picotti
Supervisor Prof. M. Heinemann Assessment Committee Prof. D.J. Slotboom Prof. M.W. Fraaije Prof. P. PicottiTo my parents and my brother
Supervisor Prof. M. Heinemann Assessment Committee Prof. D.J. Slotboom Prof. M.W. Fraaije Prof. P. PicottiTable of contents
Chapter 1 The role of fructose-1,6-bisphosphate in metabolic regulation 9 Chapter 2 Fructose-1,6-bisphosphate might regulate hexokinase activity
indirectly via chelating metal ions
33
Chapter 3 Conformational changes of enzymes observed upon fructose-1,6-bisphosphate addition are not due to a direct interaction
53
Chapter 4 Comprehensive biochemical assessment of the interaction between the flux-signaling metabolite fructose-1,6-bisphosphate and the bacterial transcription factors CggR and Cra
73
Chapter 5 Conclusions and outlook 105
Chapter 6 Summary Samenvatting Resumo 111 115 118 Acknowledgements 121
