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University of Groningen Extracellular Vesicles for Intracellular Drug Delivery Joshi, Bhagyashree

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University of Groningen

Extracellular Vesicles for Intracellular Drug Delivery Joshi, Bhagyashree

DOI:

10.33612/diss.161599194

IMPORTANT NOTE: You are advised to consult the publisher's version (publisher's PDF) if you wish to cite from it. Please check the document version below.

Document Version

Publisher's PDF, also known as Version of record

Publication date: 2021

Link to publication in University of Groningen/UMCG research database

Citation for published version (APA):

Joshi, B. (2021). Extracellular Vesicles for Intracellular Drug Delivery. University of Groningen. https://doi.org/10.33612/diss.161599194

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Extracellular Vesicles for Intracellular

Drug Delivery

Bhagyashree Sunil Joshi

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The research described in this thesis was performed collectively at the Department of Biomedical Engineering (BME) and Biomedical Sciences of Cells & Systems (BSCS), University Medical Center Groningen (UMCG), University of Groningen (RUG). This PhD work was financially supported by the Dutch Technology Foundation TTW, which is part of the Netherlands Organization for Scientific Research and partly funded by the Ministry of Economic Affairs. Part of the work was funded by Stichting Techniek en Wetenschap (STW) and de Cock-Hadders Stichting. The individual NAMASTE fellowship was funded by the Erasmus Mundus India-EU mobility consortium.

Extracellular Vesicles for Intracellular Drug Delivery

Ó Bhagyashree Sunil Joshi

The printing of this thesis was financially supported by: W. J. Kolff Institute (WJKI) at the university of Groningen. University Medical Center Groningen (UMCG)

University of Groningen (RUG)

Printed and published by: Ridderprint, ridderprint.nl

Cover design: Bhagyashree S. Joshi

Lay-out: Bhagyashree S. Joshi

Paranymphs

Abhijeet Pataskar Amrita Singh

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Extracellular Vesicles for

Intracellular Drug Delivery

PhD thesis

to obtain the degree of PhD at the University of Groningen

on the authority of the

Rector Magnificus Prof. C. Wijmenga and in accordance with

the decision by the College of Deans.

This thesis will be defended in public on

Monday 29 March 2021 at 14.30 hours

by

Bhagyashree Joshi

born on 30 May 1991 in Pune, India

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Supervisors

Dr. I.S. Zuhorn

Prof. S.C.D. van Ijzendoorn

Assessment Committee

Prof. U.L.M. Eisel Prof. R.M. Schiffelers Prof. A.J. Moshage

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Chapter 1 General Introduction and Scope of Thesis

7

Chapter 2 Endocytosis of Extracellular Vesicles and Their Cargo

Release from Endosomes

21

Chapter 3 Converting Extracellular Vesicles into Nanomedicine:

Loading and Unloading of Cargo

77

Chapter 4 DNAJB6-Containing Extracellular Vesicles as Chaperone

Delivery Vehicles: A Proteomic Analysis

135

Chapter 5 DNAJB6-Enriched Exosomes Decrease Polyglutamine

Aggregation in in vitro and in vivo Models of Huntington’s

Disease

163

Chapter 6 Heparan Sulfate Proteoglycan-Mediated

Dynamin-Dependent Transport of Neural Stem Cell Exosomes in an

in vitro Blood-Brain Barrier

195

Chapter 7 Summary and Future Perspectives

223

Appendices

233

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